We narrowed to 958 results for: Cre

...the group mentoring format Adding peer advice increases the diversity of input and perspectives Provides...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

...germane cognitive load refers to the work put into creating a permanent store of knowledge (a schema).”  In...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...

...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...

... sometimes at odds with the space needed to be creative. “You need a lot of time to let you mind process...Sometimes finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty...

...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach...

...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations...

...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

... off-target effects Prolonged Cas activity can increase the chance of off-target editing particularly ...Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem...

...The Bethune Lab has overcome this limitation by creating Split-BioID, a spatiotemporally controllable version... are expressed in cells via plasmids or by the creation of stable cell lines and these cells are then ...

...participants can identify you. You may also wish to make creative use of a footer in your poster. This is a section...scientific poster, you shouldn’t hesitate to be creative. After all, your poster is a representation of...

...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

...resulted in a cell migration defect. They have created a circRNA website so scientists can view public...developments. Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system....