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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 962 results for: Cre

Showing: 721 - 740 of 962 results

  3. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -...

  5. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with...

  6. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations...

  12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

  13. dTAG - You're it!

    Type
    Blog Post
    ...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...

  14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

  15. To Each HIS Own

    Type
    Blog Post
    ...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

  16. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...germane cognitive load refers to the work put into creating a permanent store of knowledge (a schema).”  In...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...

  17. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

  18. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  19. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...
Showing: 721 - 740 of 962 results