We narrowed to 961 results for: Cre

... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -...

...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...

...the group mentoring format Adding peer advice increases the diversity of input and perspectives Provides...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan...

...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach...

...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...

... sometimes at odds with the space needed to be creative. “You need a lot of time to let you mind process...Sometimes finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty...

...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations...

...germane cognitive load refers to the work put into creating a permanent store of knowledge (a schema).”  In...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...

...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

...The Bethune Lab has overcome this limitation by creating Split-BioID, a spatiotemporally controllable version... are expressed in cells via plasmids or by the creation of stable cell lines and these cells are then ...

... off-target effects Prolonged Cas activity can increase the chance of off-target editing particularly ...Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem...

...participants can identify you. You may also wish to make creative use of a footer in your poster. This is a section...scientific poster, you shouldn’t hesitate to be creative. After all, your poster is a representation of...

...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...