Components of CRISPR/Cas9
Type
Blog Post
...xCas9 is a variant with increased PAM flexibiliy and eSpCas9/SpCas9-HF1 have increased targeting specificity...
We narrowed to 969 results for: Cre
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Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements... -
Management for Scientists: Delegating is Key
TypeBlog Post...leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction...500bp to 10kb, with the 3.0kb fragment having increased intensity to serve as a reference band. The uncut... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post...genes with plasmids on Addgene.org AGO1 BRCA1 CREBBP CUL1 H2AFX H2AFY H3F3A H3.1 H3.3 HIST1H1E HIST1H2BB...Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin... -
A Deep Dive into BioBuilder
TypeBlog Post... college teaching experience, Kuldell sought to create a program that would capitalize on the excitement...to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...Immuno-Oncology”) is exploding. Researchers are increasingly able to harness the body’s immune system to ...microenvironment Tumor Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...the number of fluorescent proteins is steadily increasing. Often, the choice for a fluorescent protein ...of a fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with... -
Plasmids 101: Yeast Vectors
TypeBlog Post...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...the group mentoring format Adding peer advice increases the diversity of input and perspectives Provides...Have each person write a Development Plan. Set concrete goals with input from the group to make the plan... -
Antibodies 101: Fab Fragments
TypeBlog Post...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com. Why Fabs? Antibody fragments... -
To Each HIS Own
TypeBlog Post...function, however longer histidine strings can increase that risk. Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... -
dTAG - You're it!
TypeBlog Post...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...excitation light less efficiently. Similarly, decreasing the power of the excitation light reduces the... excitation/emission cycles per unit of time, decreasing the likelihood that a fluorophore will bleach... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post... sometimes at odds with the space needed to be creative. “You need a lot of time to let you mind process...Sometimes finding that uninterrupted time to be creative is challenging,” says Susan. Another difficulty...