Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 967 results for: Cre

Showing: 721 - 740 of 967 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...harness the power of light, Christopher Voigt’s lab created the first E. coli light-sensitive two-component...pathways. They also reduced system leakiness and increased dynamic range to around 100-fold. These improvements...

  2. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...leadership roles leads to more ideas, initiative and creative thinking. The team members will be more invested...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...

  4. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ... recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene expression...that stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -...

  6. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...solution is more complicated. You’ll need to be creative to be able to work with others to come up with...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... college teaching experience, Kuldell sought to create a program that would capitalize on the excitement...to SynBio Assembly Standards Find Plasmids for Creating and Controlling Gene Networks Browse All SynBio...

  10. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...must be utilized or a new combination needs to be created in advance of the experiment. The marker provided...physiological levels due to high copy numbers. This creates a potential metabolic burden on the yeast cells...

  12. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com     The modularity of antibodies...antibody and common antigen binding fragments. Created with biorender.com.   Why Fabs?  Antibody fragments...

  13. dTAG - You're it!

    Type
    Blog Post
    ...including rapidly depleting a protein-of-interest, increasing target selectivity, overcoming resistance to ...molecule binders. To overcome this limitation, we created a generalizable tag-based strategy, which we refer...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these are all...details. Or challenge yourself to think of more creative solutions. Ultimately, problem solving is less...

  16. To Each HIS Own

    Type
    Blog Post
    ...function, however longer histidine strings can increase that risk.    Number of residues Deciding... very low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended...

  17. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...delivery into recipients by conjugation. The labs screened for the host-range of the plasmids and suitability...of cells containing the particle exponentially decreases over time and the number of elapsed generations...

  18. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... or phosphate molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent...surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency. In the same vein, locked...

  19. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...germane cognitive load refers to the work put into creating a permanent store of knowledge (a schema).”  In...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...
Showing: 721 - 740 of 967 results