We narrowed to 805 results for: Eng

1. Viral Vectors 101: Chemogenetics

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   Blog Post

   ...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

2. Antibodies 101: Choosing the Right Isotype

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   Blog Post

   ...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

4. 6 Tips for Grant Writing

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   Blog Post

   ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...

5. Adapting Toehold Switches to Detect Zika Virus

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   Blog Post

   ...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

6. 10 Basic tips for mammalian cell culture

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   ...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

8. Hot Plasmids: Winter 2025

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   Blog Post

   ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

9. Common Injection Routes in Mice

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   ...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

10. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...

11. Tips to Make the Most of a Scientific Conference

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    .... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...

12. Polymerase Chain Reaction Overview and Applications

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    ...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

13. 3D Printing Meets CRISPR Cas9

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    ...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...

14. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

15. Of Myc and Men

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    Blog Post

    ...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...

16. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

17. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...

18. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...

19. Experimenting with New Careers while in Grad School

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    Blog Post

    ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

20. Genome-wide Screening Using CRISPR

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    Blog Post

    ...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...