We narrowed to 802 results for: Eng

1. 6 Tips for Grant Writing

   Type

   Blog Post

   ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...

2. Adapting Toehold Switches to Detect Zika Virus

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   Blog Post

   ...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

3. 10 Basic tips for mammalian cell culture

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   ...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

4. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

5. Tips to Make the Most of a Scientific Conference

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   Blog Post

   .... This made sure I didn’t zone out and kept me engaged throughout the day. After the conference If there...

6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...access to routinely, and it can be especially challenging when trying to develop antibodies against unstable...

7. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...standard curve, which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion...

8. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   ...Ceballos-Diaz, C., Liu, X., Paterno, G., Mejia, C., Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos...

9. Twenty Years of Addgene Sharing: CRISPR

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   Blog Post

   ... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

10. Hot Plasmids: Winter 2025

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    Blog Post

    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....

11. Common Injection Routes in Mice

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    Blog Post

    ...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

12. Of Myc and Men

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    Blog Post

    ...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...

13. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ...(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab now available as a ready-to-use viral prep ...

14. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...colleagues to reduce off-target hits in a rationally engineered Cas9 with improved specificity. Hold this flexible...

15. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...

16. Experimenting with New Careers while in Grad School

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    Blog Post

    ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

17. Genome-wide Screening Using CRISPR

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    Blog Post

    ...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...

18. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

19. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

20. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...