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  1. Pooled Library Amplifications

    Type
    Blog Post
    ...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...

  2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

  4. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

  7. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

  8. MXS Chaining

    Type
    Blog Post
    ...475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human ...Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological...

  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

  12. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  14. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  15. CRISPR Antimicrobials

    Type
    Blog Post
    ...and public health and medical organizations are working to help educate the public about proper antibiotic...observe any mutations at the target site. In a mouse skin colonization model, phagemid treatment decreased...

  16. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...library screen only to find the system itself is not working because your reporter protein doesn’t function ...Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid system. Nature Methods...

  17. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...

  18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...

  19. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  20. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...positive control will confirm that the procedure is working and should always be included.  For a negative...
Showing: 721 - 740 of 926 results