We narrowed to 1,015 results for: LEA
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TypeBlog Post...issues as one doing experiments in France. We can learn with each other through globalized, collaborative... Additional Resources on the Addgene Blog Learn How You Can Use Video to Share Your Research 5 Reasons...
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Tips for Improving Your Next Manuscript
TypeBlog Post...last, homework assignments. The instructors are leaders in the field of microbiology with multiple high-profile...groups, we were required to critique two of our colleagues’ manuscripts and discuss changes to particular... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...for scientists to understand how GPCR activation leads to downstream effects. Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...also drawn out as drugs can remain present until cleared by the body. Administering the drug once can result...vectors? Browse our Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post...work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission to make ...Other Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released transposon can now... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...choosing your perfect plasmid backbone. Or just ask – leave a message in the comments below and an Addgenie ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...cycles will keep most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast...the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...sometimes up to millions of new copies, which are released from the cell like baby spiders from an egg sac...our Viral Vectors 101 eBook, out if you want to learn more. References and resources References Balloux... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...against the variants? Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard...Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...ever like to write about a recent plasmid deposit please sign up here. Human whole genome sgRNA iBAR ...calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase that... -
Meet the New Addgene Board Members!
TypeBlog Post...Association and it was through this connection that she learned more about Addgene. “The mission to share critical...and Ear and Harvard Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has... -
RUBY-Red Siliques
TypeBlog Post...segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur at...First impressions are everything and you want to leave people remembering your potential as an entrepreneur... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city... -
K. phaffii: Rising to the Occasion
TypeBlog Post...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral...