We narrowed to 1,028 results for: LEA

1. Addgene's Top Blog Posts from 2020

   Type

   Blog Post

   ...this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this...

2. Fluorescent Proteins 101: History of Fluorescent Proteins

   Type

   Blog Post

   ...one is right for you, so if you’re interested in learning more about fluorescent proteins, I invite you ...you to download our Fluorescent Protein eBook and learn much more! ...

3. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

   Type

   Blog Post

   ...2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular...

4. Editor's Choice, August 2016

   Type

   Blog Post

   ...number of out of office responses I’ve received). Clearly we have all of our writers to thank for the excellent...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...

5. A new platform for fast functional characterization of potentially cancerous mutations

   Type

   Blog Post

   ...plasmids from this study with Addgene. Read on to learn more about their work, and how these plasmids can...PMC4737758. Additional resources on the Addgene blog Learn about our cancer collection pages Read all cancer...

6. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

   Type

   Blog Post

   ...publication. As you develop and confirm new gRNAs, please consider submitting their sequences (and plasmids...press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR Software ...

7. Who Gives a Tweet? 9 Facts About Scientists on Twitter

   Type

   Blog Post

   ...hoped to use Twitter professionally to network, learn more about alternative careers for scientists, and...to parity - the smaller the number, the more it leans towards males). This ratio is more skewed towards...

8. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   ...at a frequency of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system....

9. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   ...National Academy of Sciences in March, he and his colleagues took another important step forward by dissecting...our understanding and treatment of cancer. It is clear that one needs to capture a diverse range of CTCs...

10. Understanding GPCR Signaling Using TRUPATH

    Type

    Blog Post

    ...for scientists to understand how GPCR activation leads to downstream effects.  Bryan Roth’s lab created...GPCR causes a conformational change in the GPCR, leading to the activation of the Gα subunit. The activated...

11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    ...also drawn out as drugs can remain present until cleared by the body. Administering the drug once can result...vectors? Browse our Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources...

12. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type

    Blog Post

    ...our Plasmids 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection...

13. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type

    Blog Post

    ...manipulation tools  Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze....  Additional resources on the Addgene blog: Learn how the CRISPR software matchmaker can help you ...

14. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    ...issues as one doing experiments in France. We can learn with each other through globalized, collaborative...      Additional Resources on the Addgene Blog Learn How You Can Use Video to Share Your Research 5 Reasons...

15. Tips for Improving Your Next Manuscript

    Type

    Blog Post

    ...last, homework assignments. The instructors are leaders in the field of microbiology with multiple high-profile...groups, we were required to critique two of our colleagues’ manuscripts and discuss changes to particular...

16. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type

    Blog Post

    ...targeted RNA knockdown, which leads to unique non-specific RNA cleavage in bacteria. In mammalian systems...

17. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type

    Blog Post

    ...work, picking up plasmid deposits, and trying to learn ways Addgene can improve in its mission to make ...Other Careers Posts Visit the Addgene Careers Page Learn about Management for Scienitsts from Addgene Executive...

18. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    ...repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released transposon can now...

19. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    ...pDEST-HisMBP). After purification the protein can be released from the resin and used for various binding or...choosing your perfect plasmid backbone. Or just ask –  leave a message in the comments below and an Addgenie ...

20. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    ...cycles will keep most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast...the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your...