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We narrowed to 1,018 results for: PLE;

Showing: 721 - 740 of 1018 results

  1. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic... particular catalog item is available for order please email [email protected] with the vector name and...

  2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...incorporate into their own labs. This week we are pleased to announce the submission of the results of our...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity...

  3. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...many different things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate...responsible for the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC ...from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied by migraines...

  4. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...about FPs than is currently represented at fpvis (complete spectral information, accession numbers, sequences...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...in the early stages of development, and there is plenty of room for improvement and elaboration.  Current...

  6. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... SARS-CoV-2 interacts with human proteins. For example, we used HEK293T cells in our first study, but ...collaborative science, and making it accessible to all people, not just scientists! *If you are requesting plasmids...plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with your order...

  8. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3.   Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2.   Improved trafficking and localization... Multiple Authors...

  11. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...have tried to modify it to improve yields. For example, transfecting the producer cell line with an expression...were always normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory...

  12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...techniques are continually being refined; it is completely plausible that scientists will eventually have...many things we can navel-gaze over from the last couple of weeks, including whether or not scientists can...history of medical research, there have always been people who unethically experiment on humans, and they ...

  13. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...

  14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ... enzymes, some of which are also functional in multiple species (read Arbor Biotechnologies' press release...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown... necessary. Konermann et al. compared CasRx to multiple RNA regulating methods: small hairpin RNA interference...

  15. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...specific region of your protein of interest. For example, if you are studying a protein that may bind a ...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...higher than that of polyclonal antibodies. For example, it takes longer to develop a hybridoma than it...

  16. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike LTRs, ITRs are...): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: ...

  17. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...researchers new to GCE aren’t able to successfully complete their experiments and many others are dubious ...universal techniques that researchers can easily implement in their own labs. They provide resources, knowledge...bulletin board on their website specifically so people can talk about GCE. In fact, their first protocol...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...cool because they can transmit information, for example, from one of the 180 regions to another or from...even further defined population of neurons. For example, say I wanted to access all the neurons that signaled... Multiple Authors...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...them an ideal delivery system for human patients. Multiple viral-based gene therapies have already been approved...

  20. How Addgene Creates Content

    Type
    Blog Post
    ... look at how we create this content, using the example of our Antibody Guide, which was published alongside...can. We even have a Slack channel just for asking people if they can write or review things and another ...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...
Showing: 721 - 740 of 1018 results