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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 928 results for: Por

Showing: 721 - 740 of 928 results

  1. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...around the band as possible. To do so, it is often important to take the excised band, lay it down on the UV...sides with the razor blade. This is especially important during the DNA purification step, as many kits... fragment. The weight of the gel is directly proportional to its liquid volume and this is used to determine...manufacturer's instructions. Note: It is usually important to determine the concentration of the DNA that...

  2. Protocol - Bacterial Transformation

    Type
    Protocol
    .... Transformation of bacteria with plasmids is important not only for studies in bacteria but also because...amplification. Higher efficiency cells are more important if you will be transforming with very small amounts...critical for Ampicillin resistance but is much more important for other antibiotic resistances. Plate some or... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

  3. Using a Light Microscope Protocol

    Type
    Protocol
    ...light to detect small objects. Two of the most important properties of a microscope are magnification (...see several pieces of information, but the most important (for now) is the magnification power, such as ...be sure to carry it with two hands - one hand supporting the base and the other holding the arm - or use...your images in the future. Conclusion Like any important laboratory instrument, you should be sure to take...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ...EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ... reading. When the droplet reading is complete, export the data from all wells as a CSV file which will...

  6. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...desired insert. If you are in this situation, it is important to treat the digested vector backbone with a phosphatase...setting up the ligation reaction itself, it is important to determine the amount of cut insert and vector...being sure to increase the amount of buffer proportionally. 1μL of ligase should be sufficient for larger...

  7. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... be in the bottom portion of the gel, but all of the EtBr will be in the top portion and your bands will...overboil the solution, as some of the buffer will evaporate and thus alter the final percentage of agarose...

  8. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...CI) Heat-inactivated FBS Polybrene (10 mg/mL), Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium...of cell death upon antibiotic selection. It is important to monitor these cells regularly and replace the... the surviving cells in the culture, so it is important to do regular media changes and maintain optimal...

  9. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...vector cores. Our titers were similar to those reported by these institutions. This protocol is for a ...of causes, such as pipetting error and sample evaporation. Make the master mix after all the samples have...plasmids to find a suitable one. Some labs have reported better results when the plasmid is linearized....

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...transduction efficiency by facilitating nuclear import of the vector’s preintegration complex in the transduced...below. Do not change the ends; these bases are important for cloning the oligos into the pLKO.1 TRC-cloning...temperature. This will take a few hours, but it is important for the cooling to occur slowly for the oligos...you have identified the positive clones, it is important to verify the insert by conducting a sequencing...

  11. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations...well ventilated area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2...

  12. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Background Information Bacterial glycerol stocks are important for long-term storage of plasmids. Although you...completely and will improve the shelf life. It is very important that you shake the glycerol before freezing (5...

  13. Water Bath Protocol

    Type
    Protocol
    ...on the surfaces of the water bath as the water evaporates from the tub. Disinfectant may be added to prevent...cover on the top of the water bath to prevent evaporation and maintain the desired temperature. This also...

  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

  16. Centrifugation

    Type
    Protocol
    ...represent the solids in your sample while the liquid portion is known as the supernatant. Depending on your ...supernatant, or both. Conclusion Centrifuges are important tools for many different protocols. Improper use...

  17. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers...

  20. Gibson Assembly Protocol

    Type
    Protocol
    ...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...
Showing: 721 - 740 of 928 results