Plasmids 101: Repressible Promoters
Type
Blog Post
... by Mary Gearing in 2018, and updated by Hannah Dotson in Dec. 2022.
References and Resources
References...
We narrowed to 733 results for: SON
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Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...GRSMs, we recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post..., Xie X, Huang R, Sun Y, Sun H, Zhang J, Xu Y, Songyang Z, Huang J (2016) Efficient Production of Gene-Modified... -
Plasmids 101: Restriction Cloning
TypeBlog Post...note that UV light can damage DNA and that proper personal protective equipment should be worn when visualizing... -
Antibodies 101: Validation
TypeBlog Post....1038/518027a Roncador G, Engel P, Maestre L, Anderson AP, Cordell JL, Cragg MS, Šerbec VČ, Jones M, ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post... Jason Nasse... -
Finding Your Science Policy Path
TypeBlog Post... important, but must be taken through your own personal lens. For me, the Veterans Health Administration... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes ... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...effectively than Cas9 complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...neuroscience community like wildfire and for good reason. For the first time in the history of neuroscience... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...25578634. PubMed Central PMCID: PMC4412587. 28. Mohsen-Kanson, T., et al., Differentiation of human induced pluripotent... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...of transferrin receptor CD71 in HeLa cells. Comparison of photoactivatable CRISPR strategies Publication... -
Technical Design of a Western Blot
TypeBlog Post... Rachel Leeson...