Lab Automation at Addgene
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Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...more about precise neuronal targeting using AAV technologies Browse all of our viral vector blog posts Resources... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...and not targeted to a specific area. Some other technical considerations for stereotaxic injections are:... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...keynote speech and in the workshops on CRISPR techniques and new tools for conditional expression and ... -
A Look at Addgene's QC Process
TypeBlog Post...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post... enables rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...genomic landscapes should be editable with CRISPR technology. Fig. 1: SpCas9 has sparse PAM coverage... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods Many calcium reporters use a single fluorescent... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest to MoClo ... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of FPs as well... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...papers, and more. Saving papers There isn’t technically a way to save papers on Twitter, but you can ... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...Massachusetts Boston Christine Oslowski, PhD - Biotechnology Communications Specialist at AsisChem Inc. and... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...of lentiviral vector titration methods."BMC biotechnology 6.1 (2006): 1. PubMed PMID: 16836756. PubMed... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...flexibility in behavioral experiments. However, the technical demands of using these wireless devices are significantly... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...Postdoctoral scholars should have the skills and techniques needed to facilitate effective teamwork, manage... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...lab mates, and PI can provide feedback on your technical presentation, but those outside of your field ... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...goes into a 96-well plate and comes out of it. Biotech companies are known to have tools that do this ... -
Hot Plasmids: Summer 2025
TypeBlog Post...hinders their use for long-term imaging or advanced techniques like super-resolution microscopy. To make this...