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We narrowed to 1,042 results for: URE

Showing: 721 - 740 of 1042 results
  1. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...stages (i.e. postdoc level) and can include the pressure to publish in high-impact journals, and the requirement...with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech companies), and...company, less exposed to the fast dynamics and pressure which are often found in sales or lab-based roles...name a few). Depending on the particular company structure you may be able to move into one of these departments...
  2. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... new experimental species. Discovering ways to culture, propagate, transform, store, and share all of ... "for development of tools, approaches, and infrastructure necessary for direct tests of cause and effect...was excited to be invited to attend. My job is to ensure that, in general, these labs all include materials...from Old Faithful in 60 °C soils. Dendrobatidae (Lauren O’Connell)  Poison frogs are great for studying...
  3. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...sent to the AbuGoot lab for testing, they weren’t sure what they would find… but it was safe to say the...dual activities: processing pre-CRISPR RNA into mature CRISPR RNA and cleaving RNA at positions defined.... But they weren’t done engineering quite yet – surely, they thought, the integration rate could be improved...with the single-protein CRISPR effector Cas7-11. Nature. 2021 Sep;597(7878):720-725. doi: 10.1038/s41586...
  4. March for Science

    Type
    Blog Post
    ...Makes Better Science Nature: What Trump’s new travel ban means for science Nature: Meet the scientists...job of having evidence”, scientists want to make sure having evidence is the job of everyone in government...all you need to do is select your state. State Legislatures: To find the website to contact representatives...
  5. Five Popular Model Organisms

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    Blog Post
    ... than studies in other model organisms or cell culture. For example, Scott Lowe’s lab utilized mice to...double-stranded RNA in Caenorhabditis elegans." nature391.6669 (1998): 806. PubMed PMID: 9486653. Hesselson... and its relationship to the human genome." Nature496.7446 (2013): 498. PubMed PMID: 23594743. PubMed ...organism for studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et ...
  6. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ...painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene, and may have...provide enough background for you to distinguish the features of any common lab strain and determine whether... DNA, and more. Many mutations that make these features possible are present in most commercial strains...of protein expression in E. coli and the common features found in those bacteria. Check our our blog post...
  7. Plasmids 101: Repressible Promoters

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    Blog Post
    ... either category, but those are a subject for a future post! How are repressible promoters regulated...commonly used in yeast. In the first 24 hours of culture, when glucose is abundant and ethanol concentrations...during the late ethanol consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed...
  8. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...experimental results and 2) perform targeted off-target measurements at those sites to get an idea of how high the...effects, you still need to do your due diligence to ensure they are indeed a non-issue. Above we mentioned...these outcomes. Rigorous analysis is essential to ensure off-target events are below a given threshold for...your risk level is, how to lower it, and how to ensure off-targets don’t mess with your results when they...
  9. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...contest is over, we will host other contests in the future. Subscribe to the blog to stay in the know!   ... hunt works works: You will be trying to find pictures of Addgene pets with Addgene-branded clothing ...pet gives you a clue to help you find the next picture. If you find the next correct photo, you will see...
  10. The Twelve Days of CRISPR

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    Blog Post
    ...Addgene gave to me: a method to capture promoter-enhancer 3D chromatin structures using CRISPR. On the eleventh... you’ve been enjoying our CRISPR blog series. Be sure to subscribe to the CRISPR blog posts so you don...
  11. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...flick the tube several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything... the latest episode.  Have some suggestions for future tips?  Send your ideas to [email protected].  Additional...
  12. Bringing Renewable Energy to Addgene

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    Blog Post
    ... or hydroelectric in nature. The operation of -80C and -20C freezer temperatures, biosafety cabinet ventilation... lighting/HVAC requires a significant energy expenditure here at Addgene. These are critical systems that...
  13. PEI Calculator for Planning AAV Packaging Transfections

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    Blog Post
    ...key step to any experiment and can help prevent future headaches. To help you plan PEI transfections for...simple to make adjustments for the type of tissue culture dish and number of dishes used. Here’s to making... pHelper: 2 RepCap Select what type of tissue culture dish you will be performing the transfection in...
  14. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

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    Blog Post
    ...What's a lab group in industry? The collaborative nature of working in industry and how delegating can empower...success. The importance of thinking about the big picture and not just the next experiment. Chapter 6: Advice... the treatment of various diseases, from heart failure to Alzheimer’s.  You can follow her on Twitter ...
  15. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...29:54 - 31:25 : Nature's defenses against gene drives. 31:26 - 33:08 : Plans for future work at the Wyss...advisory board where he will help shape Addgene's future. Listen to this episode for insights into the process...
  16. Countdown to Halloween @Addgene

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    Blog Post
    ...for years – searching our photo archives I found pictures back through 2009, though I suspect that our costume...months in advance. Our teams comb the news (pop culture events of the past year obviously make for good...takes home the ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among...
  17. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...what the founders hope to see in the repository’s future. If you’ve ever been curious about what it takes...success Chapter 6 - Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization...Tufts Launchpad, and more Chapter 10 - Addgene's Future, the research team, and the importance of sharing...
  18. Performing In Vivo CRISPR Screens Using the FITS Approach

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    Blog Post
    ...perform in vivo screens in other cell types. A key feature of this system is a lentiviral gRNA expression ...the statistical power of in vivo screens. Figure 1: Using FITS to perform in vivo CRISPR screens...clonal dynamics in lymph nodes (LN) and tumor cells. Figure used with permission from Milling and Markson et...
  19. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

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    Blog Post
    ...and look forward to hearing from her in the future. Future awardees We will be opening the Addgene/Seeding...them the most. Check back here to learn more about future awardees and their inspiring research. Additional...
  20. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...and proteins associated with pathogenesis. In his future work. Dr. Bengyella would like to use the expertise...to do excellent work in the biological sciences. Future awardees Now that this first round of awards has...enable scientists in developing regions in the near future. Additional Resources Apply to Recieve Equipment...
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