We narrowed to 772 results for: Vars;

1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ...focus on what makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a:...

2. Fluorescent Proteins 101: Aptamer Fluorophores

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   Blog Post

   ...for biosensors. Aptamer biosensors are used in a variety of applications, including detection of environmental...

3. How to Write a Scientific Review Article

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   Blog Post

   ... class of proteins was mutated, as reported in various studies. We made a table that listed each protein...

4. Bioinformatics at Addgene

   Type

   Blog Post

   ...re-use or borrow existing code and control how the various pieces were connected, as this enabled us to rapidly...

5. New CRISPR Tools: Cas7-11 and PASTE

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   Blog Post

   ...then used this information to identify a large variety of related bacteria with similar Cas systems. Some...

6. Viral Vectors 101: Pseudotyping

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   Blog Post

   ...cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein...

7. Neuronal labeling with Spaghetti Monster

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   Blog Post

   ...detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009):...

8. Analyzing CRISPR Editing Results with ICE from Synthego

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   Blog Post

   ...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   Blog Post

   ...karyotypically normal and are able to differentiate into various cell types of all three germ layers [7]. To further...

10. Viral Production at Addgene

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    Blog Post

    ...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ...

11. QC Sequencing Technologies at Addgene

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    Blog Post

    ...Addgene have minor differences due to in silico variations and lack of verification. To address this issue...

12. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis...

13. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...packages needed to travel meant the accessibility varied country to country.   Figure 1: Number of countries...

14. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...simple and easy technique to implement. Success might vary between experimenters. Vacuum infiltration (Mortensen...

15. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post

    ...manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the...

16. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post

    ...some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti...

17. Supporting Reproducibility with a Connected ELN

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    Blog Post

    ...document is edited, and it’s possible to link between various documents. A second example is the integration ...

18. Tips for Writing a Good Cover Letter

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    Blog Post

    ...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ...bacteria. They consist of a toxin which can affect a variety of cellular processes and an antitoxin that suppresses...

20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...ended up being very straightforward to engineer various cell lines from different tissues and mammalian...