Plasmids 101: Protein tags
Type
Blog Post
...between column runs. The TEV protease itself, with various mutations used to increase its stability activity...
We narrowed to 776 results for: Vars;
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Neuronal labeling with Spaghetti Monster
TypeBlog Post...detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009):... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...karyotypically normal and are able to differentiate into various cell types of all three germ layers [7]. To further... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...focus on what makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a:... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...for biosensors. Aptamer biosensors are used in a variety of applications, including detection of environmental... -
How to Write a Scientific Review Article
TypeBlog Post... class of proteins was mutated, as reported in various studies. We made a table that listed each protein... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...organism. As for multi-drug resistance: definitions vary in the literature, but generally organisms with ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...Reference References del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...the Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...simple and easy technique to implement. Success might vary between experimenters. Vacuum infiltration (Mortensen... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of Cargo) delivers Cas9 RNP to the... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...document is edited, and it’s possible to link between various documents. A second example is the integration ... -
Bioinformatics at Addgene
TypeBlog Post...re-use or borrow existing code and control how the various pieces were connected, as this enabled us to rapidly... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...then used this information to identify a large variety of related bacteria with similar Cas systems. Some... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...packages needed to travel meant the accessibility varied country to country. Figure 1: Number of countries... -
Tips for Writing a Good Cover Letter
TypeBlog Post...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is... -
Viral Production at Addgene
TypeBlog Post...control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ... -
QC Sequencing Technologies at Addgene
TypeBlog Post...Addgene have minor differences due to in silico variations and lack of verification. To address this issue...