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TypeBlog Post...CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation with...decaffeination operon Article contributed by Angela Kaczmarczyk Listen to the synthetic decaffeination operon...
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Lab Automation at Addgene
TypeBlog Post...ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find ...Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at Addgene.org Find... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...gleaned from the literature and engagement with researchers who study the protein or structure of interest...introducing tags into your gene of interest. Researchers have developed a variety of endogenous gene tagging... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...This post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina ...Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment... -
Plasmids 101: FLEx Vectors
TypeBlog Post...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...Eric Green, Director of the National Human Genome Research Institute (NHGRI) of the NIH, started off with...acid science still has many surprises for the researchers in this field. Resources at Addgene Browse ... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ... -
The Materials Science of Optogenetics Experiments
TypeBlog Post... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post... of a gene exists, there must be assays that researchers can use to quantify said copies. They do so using...commonly available and may not be an option to all researchers. Accuracy needed Do you need to call exact... -
Transferable Skills: Negotiation
TypeBlog Post...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...set out to fill this gap by highlighting that researchers learn many transferable skills while working ... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand... -
10 Basic tips for mammalian cell culture
TypeBlog Post...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers... Addgene.org Find mammalian plasmids for your research Find viral vectors for infecting mammalian cells... -
Transferable Skills Guide: Managing a Team
TypeBlog Post... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier... -
Antibodies 101: Flow Compensation
TypeBlog Post...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...