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  1. RNA Extraction Without A Kit

    Type
    Blog Post
    ...something that broke down RNases before RNases had a chance to break down RNA upon cell lysis.  In the late...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

  2. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    ..., and is long enough that there is virtually no chance of it occurring randomly. Therefore, inserting ...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    ...edited and the sequence of the edit, so its design changes for every new prime editing application. And optimizing... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...nucleotides are represented equally, that’s only a 1/16 chance of having a PAM at a given site. While it is possible...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...was limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12...and screening that led to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco...

  8. CRISPR in the Clinic

    Type
    Blog Post
    ...polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of the TTR protein, which contributes...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding and therefore..., P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques ...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...confluent and the media will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week...

  12. Lab Automation at Addgene

    Type
    Blog Post
    ...spirit include: Start using electronic multichannel and single channel pipettes. These can speed up any project...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...sequencing of neurons from each class to fully characterize the transcriptome. The first data release for...C.elegans neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle ...

  14. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...derived from random crosses between mice of uncharacterized backgrounds to create a limited but genetically...the injection of a targeting vector alone, some challenges still exist. Nucleases may cause off-target effects...

  15. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to ...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...semi-circle (so they can see each other) on comfortable chairs (stools if you have to, but this is tough for women...things started. Give a couple of audience members a chance to participate. For example, at a recent event ...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... developed in human cells, has been uniquely challenging to apply in dicotyledonous plants, or dicots....ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants...
Showing: 721 - 740 of 946 results