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Addgene

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Showing: 721 - 740 of 960 results

  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...and left undisturbed for 13 days. (a, b) Colonies formed in individual wells of a 96-well plate after expansion...monoclonal cell lines. When this protocol was performed at Addgene on the A549 cells described in our ...expression and/or other phenotypes. For example, perform Western blotting to screen for lines with the highest...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

  6. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...editing to pooled library screening. It also has information for planning your CRISPR experiment. For a quick...

  7. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

  9. Addgene at SfN23!

    Type
    Blog Post
    ... to our community.  You'll also find tons of information about Addgene's ready-to-use antibodies, viral...

  16. Deck the Lab 2022!

    Type
    Blog Post
    ...Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make ...

  17. Call for Guest Bloggers

    Type
    Blog Post
    ... One of our goals is to be a go-to source of information on recent advances in the biological sciences...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium...specific molecules or other factors. Most often, a conformational change in the sensing region of the construct...Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically...
Showing: 721 - 740 of 960 results