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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 721 - 740 of 961 results

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... minimal limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece...recipient plasmid backbone. Transformation Proceed with the transformation according to the manufacturer...from your transformation will give you the first indication as to whether your transformation worked. Your...Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile...see (Link opens in a new window) NEB for more information). Thus, we recommend that you add 3-6 bases upstream...should ensure that the bases do not result in the formation of a hairpin structure within your primer. In ...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ...our case 220 mL) to the same bottle and swirl to form a medium/agar colloid. Cover the opening of the ...lab. The addition of agar to LB results in the formation of a gel that bacteria can grow on, as they are...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...and left undisturbed for 13 days. (a, b) Colonies formed in individual wells of a 96-well plate after expansion...monoclonal cell lines. When this protocol was performed at Addgene on the A549 cells described in our ...expression and/or other phenotypes. For example, perform Western blotting to screen for lines with the highest...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

  5. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium...specific molecules or other factors. Most often, a conformational change in the sensing region of the construct...Citron/Citroff), mitochondrial or cytosolic High-Performance Intensiometric Direct- and Inverse-Response Genetically...

  10. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

  11. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...editing to pooled library screening. It also has information for planning your CRISPR experiment. For a quick...

  13. Addgene at SfN23!

    Type
    Blog Post
    ... to our community.  You'll also find tons of information about Addgene's ready-to-use antibodies, viral...

  20. Deck the Lab 2022!

    Type
    Blog Post
    ...Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make ...
Showing: 721 - 740 of 961 results