We narrowed to 968 results for: form

...hour or so, the sample is immediately ready to transform into competent cells. The master mix of enzymes...Gibson assembly master mix for 1 hour, and then transform into competent cells. The success rate of this...

...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...

...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

...also known as KDR), orchestrating blood-vessel formation. Precise, real-time pharmacology of this target...modifications to the binding sites and various isoforms led to subtle changes in co-receptor dynamics,...

... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...11%. It has higher efficiency and lower indel formation than methods relying on homology-directed repair...

...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

... studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly...to screen additional ligases, conditions, and formulations and has led to the development of the new HiFi...

...scholar/student: visiting from another university to perform research   Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career...

...and Agrobacterium-mediated transformation are available. For more information on this system, check out...

...certain circumstances) Current base editors can perform any base-to-base conversion in principle, but some...the process of double nicking increases indel formation slightly. Designing the sgRNA with a spacer that...

...Antibodies 101 blog series.  Stay tuned for more information on our blog and social media! ...

... of vectors, capsids, and tools; and helpful information on everything from producing your own vectors...

...Intro to the Lab Bench series is meant to be an informative introduction for those who are just starting ...

...topic, allowing you to easily access relevant information for planning, executing, and troubleshooting ...

...editing to pooled library screening. It also has information for planning your CRISPR experiment. For a quick...

... day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth...

...Andrea Radtke, Ziv YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors...

... to our community.  You'll also find tons of information about Addgene's ready-to-use antibodies, viral...

...value, then, in having a guide that curates this information. And so I created the Codon Guide to Synthetic...

...  We’ve been working hard to create the most informative and entertaining protocol and explainer videos...