We narrowed to 1,050 results for: ins
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TypeBlog Post...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing. Demonstrate you are open to feedback...
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X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...develop a searchable and sortable datatable that contains validated gRNA sequences. Validated in this context...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...CRISPR/Cas9 for targeted genomic deletions We were inspired to produce intrachromosomal deletions based on...copy at a given locus may be useful to study haploinsufficiency. Subsequent modifications at the remaining... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post... own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you realistic expectations...mentor does all of these things and more. It is instructive (and entertaining) to review this list of bad... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year. In September... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm... plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein... -
New Viral Vectors - Fall 2024
TypeBlog Post...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...vectors from the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium ... -
An Addgene Summer Internship
TypeBlog Post...toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp (preferably). I ...college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems...off-target effects! This characteristic probably explains why pC016 - LwCas13a guide expression backbone... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...for example, cutting open a heart to look at the inside. We do a lot of interactives and videos, as well... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post...one of the highlights of the conference. It was inspiring to see how microbiology research is improving ...symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening... -
10 Ways to Share Your Science!
TypeBlog Post...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ... -
Advice for Choosing a Research Project
TypeBlog Post... your professional brand. It offers colleagues insight into your interests and will determine how you ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive Activity through Recombinase...protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...not rare codons and many genes where there are instances of rare codons, sometimes intentionally modulating...