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We narrowed to 971 results for: lars

Showing: 721 - 740 of 971 results

  1. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based SaCas9 genome... deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse bacteria...

  2. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...researcher. The future of DayCon DayCon and other similar events show how meaningful science outreach can...event and to expand the conference to accommodate a larger number of attendees. This first-year event’s success...

  4. Editor's Choice, August 2016

    Type
    Blog Post
    ...of NgAgo In particular, we need to thank Pooran Dewari who wrote this month’s most popular blog post on...

  6. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... meeting with someone to ask about a job or a particular position at their company, do NOT call this an... is a good time to ask personal questions about salary, opportunities for advancement, challenges and ...

  7. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...in open science this year. Here it goes, in no particular order, my top 10 open science developments, 2013...-respected and high-profile universities. We particularly recommend Eric Lander's intro to biology course...

  8. Kit Free RNA Extraction

    Type
    Protocol
    ...Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This protocol describes... (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent®, RNAzol®, QIAzol® ...Homogenize or lyse tissues or cells in TRIzol® or a similar product. For tissues: use 1 mL of TRIzol® per 100...

  9. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs...programs SITN runs and describes some of the more popular topics from the Science by the Pint series. Chapter...

  10. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that...

  13. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system is the single-color...studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It could also have...

  15. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer...the University of California, specialising in Molecular Biology. She has worked in a range of labs - cancer...

  16. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...

  17. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248...

  18. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning...
Showing: 721 - 740 of 971 results