We narrowed to 810 results for: mal

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   ...allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA...

2. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...inhibit the S. aureus Cas9, a Cas protein that is smaller and easier to deliver, but so far has few inhibitors...

3. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   ... often cycled between 37°C and 16°C to provide optimal reaction temps for both the restriction enzyme ...

4. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   ... number (if known), antibiotic resistance, and optimal growth temperature are listed, as well as the name...

5. Which Fluorescent Protein Should I Use?

   Type

   Blog Post

   ...optimized for 37°C, and is therefore most suited for mammalian or bacteria studies, whereas GFPS65T is better...

6. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   ...district’s science curriculum coordinator with minimal luck, but eventually the 8th grade science teachers...

7. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   ... or improved editing ability in a RESCUE system small enough for viral delivery.  Find the RESCUE RNA ...

8. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ...in science if you’re anything other than a white male. “The Nuts and Bolts” is the title of the chapter...

9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ...Select the model system for your research (e.g. Mammalian) Vector Type: What type of experiment are you ...

10. Tips for arabidopsis transformation

    Type

    Blog Post

    ...inoculated from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this...

11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ...The Root and Doench lab selected sgRNAs in this optimal window and ranked them based on their optimized...

12. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...benefits of lentiviral vectors have been observed in small trials for other diseases, including the common ...

13. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ... 2D barcode can store a lot of information in a small space and is ideal for tracking large collections...

14. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    ...strategy builds on the steps listed above and formalizes your development. You may consider earning a ...

15. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    ...serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy and things won’t always...

16. Lab to Office Culture Shock

    Type

    Blog Post

    ...work environments outside of academia provide a formal training regime and the right set of tools to help...

17. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    ...sensitivity, the DNA is first amplified through isothermal amplification by RPA. When Cas12a-cRNA base pairs...

18. Transferable Skills Guide: Creativity

    Type

    Blog Post

    ...PhDs found that many researchers feel they lack formal training in a variety of transferable skills. At...

19. Hot Plasmids - November 2023

    Type

    Blog Post

    ...transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors, but just as effective and...

20. How to Be an Excellent Trainee

    Type

    Blog Post

    ...demonstrating it. Requests like this are both very normal and very helpful; don’t be afraid to ask! Especially...