Sweating the Small Stuff: Details in the Lab
Type
Blog Post
...By being creative, thoughtful, and open to new methods, you’ll be able to find strategies and systems ...
We narrowed to 850 results for: met
-
-
Antibodies 101: Isotypes
TypeBlog Post...in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...forward. The viral vector project team develops methods, performs extensive quality control, generates ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post... your deck. If someone asks a question about something they did not understand about your presentation... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in the SynBio ... -
Clear and Accessible Writing
TypeBlog Post... team, working together to share information. Sometimes it is most helpful to use a lot of jargon and ... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post... your hits, you can easily sort them by other parameters, such as E-value for protein sequence searches... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...Prior to Tufts Health Plan, Carl worked at both MetLife & Ernst & Young, LLP, auditing insurance, finance... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...efficiency of TurboID and miniTurbo makes PL a viable method for proteomics in model organisms in which it was... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post... of unmodified proteins. Affinity purification methods typically require a protein to be tagged, which... -
A Deep Dive into BioBuilder
TypeBlog Post... they remember the moments when they discover something new for themselves and when they effectively contribute... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...equipment that still works. “We use functional spectrometers from the early 1990s that were free and free... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post... or who your new contact will connect you to. Sometimes, it is not who you know but who knows about you... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...Protein A or Protein G purification is one common method. These proteins bind the IgG heavy chain. Running... -
dTAG - You're it!
TypeBlog Post... use of these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens HRP (P) Tyramide signal...