We narrowed to 938 results for: mpl

1. Targeted Mutagenesis with EvolvR

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   ...conditions in order to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) ...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...

2. How Addgene Creates Content

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   ... look at how we create this content, using the example of our Antibody Guide, which was published alongside...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent protein...eukaryotic cells via a virus or viral vector. One example are bacteriophages that attach to bacterial membranes...

4. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   ...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...

5. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   ...that share a similar direction or problem. For example, a group of people who are all interested in making... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or...

6. Will You Be My Mentor? Finding and Asking for Mentoring Support

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   ...you work with Give more informed feedback (for example, they may be able to hear you present or see your...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

7. Summer SciComm: Preprints

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   ...reluctance to share their work too openly too soon or simply a lack of awareness of the benefits of preprints...can! At the time of writing, Mike Lacy was also employed by the American Society for Cell Biology in a ...

8. What's New in CRISPR - November 2021

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   ...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...greatly impacting the level of gene expression. For example, the synthesis of deoxychromoviridans requires ...products is needed, such as within a multi-protein complex, stabilized promoters can eliminate variability...

10. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...250 nm), and studying endocytosis is further complicated by the reliance on pH-based biosensors used to...rapid and reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying...

11. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...accessible, anyone could present the material. For example, Addgene’s contributions by Tyler Ford included... and matched to serve a specific audience. For example, I joined this effort later in the year and in ...

12. CRISPRainbow and Genome Visualization

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    ...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...

13. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...

14. 5 Steps to Writing Grant Proposals for Grad Students

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    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse transcriptase...

16. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences...

17. Progress Towards a PAM-Free CRISPR

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    ...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...engineered Cas9 with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by...

18. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...being a grad student to an employee, and being treated like an actual employee, was really reaffirming.”...

19. Early Career Researcher Toolbox: Finding Relevant Papers

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    ...more about authors by exploring author pages (for example, Melina Fan’s author page). These pages show all... Key features Setting up a personalized feed is simple. You can enter as many search terms as you like...

20. Cpf1 Update: Comparison to Cas9 and NgAgo

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    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...