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  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

  6. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...engineered Cas9 with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

  9. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated...modular and flexible system can be used to construct complex lentiviruses for modification of mammalian cells...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...more about authors by exploring author pages (for example, Melina Fan’s author page). These pages show all... Key features Setting up a personalized feed is simple. You can enter as many search terms as you like...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... Reddit AMA Perhaps we should start with some examples of a bad panel discussion? You know, the one where... audience members a chance to participate. For example, at a recent event on nonprofit science organizations...

  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  17. Common Injection Routes in Mice

    Type
    Blog Post
    ...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

  20. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...attention on social media. I gained a concrete example to use to promote future attempts and learned a...roles of scientists outside of academia One recent example: Don't call me a dropout: Why science needs more...
Showing: 721 - 740 of 929 results