We narrowed to 941 results for: omp

1. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   ...would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation ...and colonization opportunities for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach...

2. Visualizing Genomic Loci with CRISPR-Sirius

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   ...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...

3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   ...techniques are continually being refined; it is completely plausible that scientists will eventually have...standardized process of good research practice: computer simulations, in vitro research, animal models,...

4. Networking Follow-up: I Met a Person. Now What?

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   ...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

5. With Our New Viral Service, We're Taking CRISPR Further!

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   ... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

6. Four Factors that Differentiate the Stem Cell Field

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   ...field. Of course they are competing with each other, but it's a healthy competition. What an honor for Addgene...

7. From Lab Bench to Leadership: Transitioning into Management as a PhD

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   ...and learn from them. I started at my previous company by taking on responsibilities such as mentoring...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

8. Viral Vectors 101: Viral Applications

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   ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

9. Sweating the Small Stuff: Details in the Lab

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   ... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...several steps into one. Soon you’ll figure out what prompts you need to consistently and accurately run a protocol...

10. Antibodies 101: Isotypes

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    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

11. GPCRs: How Do They Work and How Do We Study Them?

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    ...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

12. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    ...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...resulting in a Cas9 with improved specificity. They compared on-site and off-site activity of Sniper-Cas9 to...et al. "Evolved Cas9 variants with broad PAM compatibility and high DNA specificity." Nature 556.7699 (...

14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    ... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

15. Top Requested Lentivirus and AAV of 2016

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    ...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...

16. Plasmids 101: Screens vs. Selections

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    ... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...

17. Hot Plasmids - May 2022

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    ...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

18. Plasmids 101: Methylation and Restriction Enzymes

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    ...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

19. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    ... Provide perspective on core values at another company/department Give more candid feedback and be able...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

20. Addgene: The First Twenty Years

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    ...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids, was shared with researchers ...