We narrowed to 985 results for: por

... perfect Our first tip, and arguably the most important, is to take some time to sit down, plan your cloning... plates of frozen glycerol stocks. It is very important that these stay frozen at -80 °C, since multiple...

...been known about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied ...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...protein attaches to cell surface receptors, other important binding events take place during the course of...

...fusion based on your experimental needs. Equally important, before cloning you must consider whether and ...potentially affect function [5]. We and others have reported that FP cysteine residues form inappropriate disulfide...

...added to the growth medium. Induction timing is important, as you typically want to make sure your cells... optimal because of the high cell-density they support; however, minimal media supplemented with M9 salts...

... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

... to therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

... areas of microbial research like metabolism. Transport, biofilms, and much more. Because of it’s BSL-...designed for use in Arabidopsis. There are also important tips for plant transformations and the use of ...

...1964 . https://doi.org/10.1172/jci34316 Bak RO, Porteus MH (2017) CRISPR-Mediated Integration of Large ...Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064...

...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

...cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an antigen of interest...interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction and release...

... out of fashion but is still useful and most importantly, effective. In the research world, there is a...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...

...purification and minimizes sample loss while also supporting imaging, making it easy to identify dozens or...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...ratios of recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance...

...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...ratios of recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance...

...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

...system to modify your target DNA, you first electroporate a linear donor DNA substrate (either dsDNA or...Expression of lambda red recombination genes. Electroporation of substrate DNA and outgrowth of bacteria....

...around the band as possible. To do so, it is often important to take the excised band, lay it down on the UV...sides with the razor blade. This is especially important during the DNA purification step, as many kits... fragment. The weight of the gel is directly proportional to its liquid volume and this is used to determine...manufacturer's instructions. Note: It is usually important to determine the concentration of the DNA that...