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We narrowed to 1,046 results for: sta

Showing: 721 - 740 of 1046 results

  1. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ..., does the fluorescent tag need to be pH stable or withstand a particular solute concentration or temperature...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally...

  2. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...during an initial fixation step. This serves to stabilize DNA-protein interactions for subsequent sheering... of the limitations of ChIP-seq. CUT&RUN, which stands for ‘Cleavage Under Targets and Release Using Nuclease...likely that this technique will quickly become a standard method alongside ChIP. Many thanks to our guest...

  4. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...work.  5. Do you enjoy bench work? It’s easier to stay at the bench longer (or for your whole career) with...often technical career tracks for PhDs who want to stay at the bench. So if you enjoy wielding a pipette...hopefully the questions in this blog post can help you start to figure out if a master’s is a good fit with your...

  5. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...down the hall who got it from some technician downstairs who got it from who knows where), now is as good...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...

  8. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...alone.  And a longstanding inclination to be a teacher was looking for an outlet. I started asking people... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...

  9. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ... the fusion protein includes an AviTag and a 6X HisTag for affinity purification (see plasmid map and ...dramatically increased accessibility of the AviTag and HisTag, which were “buried” in a few of our proteins that...proteins - but it would remove both the AviTag and HisTag. We were surprised that we couldn’t find an all-in-one...

  10. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...limitations because it turns a dynamic process into a static measurement and it provides limited information...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...in acidic environments, while mRFP is relatively stable and fluorescences even at the acidic pH found in...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    ..., most of the nucleic acid transfected will not stably integrate (unless you used a transposon system,... think. We have protocols available to get you started and information on the specific viruses available... Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage...

  12. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...biology tool. These systems allow researchers to understand bacterial gene clusters that produce useful natural...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...the 3rd edition has now been released), and have started providing recorded interviews with Addgene community...and actionable content in the coming years as we stay on top of the many developments in science and the...give researchers the background they need to get started using plasmids and the revolutionary CRISPR technology...

  15. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...hACE2 transgenic mouse strain, for example, is sustained by the availability of this strain from The Jackson...Laboratory. Originally developed and characterized by Stanley Perlman, of the Carver College of Medicine and ...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

  16. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...about biology in high school, it wasn’t until I started working in my first molecular biology lab in college...into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production proceeds... 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...

  17. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...help me get the engagement I wanted. I therefore started planning a game that would force my students to...and strategy skills to apply those concepts and understand how therapeutic strategies work. Find Addgene...Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer...

  19. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... treated HeLa cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase...retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...vitro and in living organisms to better study and understand these diseases and disorders. You can find the...

  20. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    .... In her graduate work in Michael Lin’s lab at Stanford, Xin Zhou greatly expanded that list by using ...graduate student in Thomas J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based...throughout her training and wants to make her work understandable to people both inside and outside the scientific...
Showing: 721 - 740 of 1046 results