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...vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview...organoids. The Organoid EasyTag System is a straightforward workflow for doing gene targeting, reporter...specificity for SLs in vitro, and they further demonstrated that when tested in tobacco protoplasts, the...

...Stories and Strategies for PhDs Making the Leap to Industry." He successfully transitioned to industry...breakthroughs often occur when the unexpected infiltrates the realm of focused exploration. Here, we delve...that may benefit your current project. Another strategy could entail immersing yourself in diverse literature...viewpoints influence our encounters. It underscores the contrast between perceiving the world solely through logic...

...their goal of PhD training when selecting a research topic. The end point of training is not to become ... the field? What are possible pitfalls in your strategy and how can you mitigate them? If you were to ...become a pro in a field, but to be trained to think and perform like a scientist. Researchers should  pick ...before diving in. Research projects are designed to train graduate students and postdocs to think analytically...

... CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening viruses. Evolving to...too, are famous for their ability to devise new strategies to reproduce within a host cell. A few virus ...Surprisingly, few genes have been identified that neutralize CRISPR/Cas. Earlier this year, expression of ...only deactivate the Type I-F system and are not translatable to the other CRISPR subtypes. Still, the large...well-suited for various innovative biological and translative technologies. Addgene has empowered researchers...

... our values of transparency, reproducibility, and community-building. The transparency begins with sharing...share plasmids with each other. Together, we can transform the antibody sharing landscape as well.     References...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD... Improving Reproducibility through Rigor and Transparency of Experimental Methods. Neuron 90(3):434-6....

...sequences is Proteobacteria - the most frequent transfer of genes is from Proteobacteria to mammalian expression...natural gene sequence is, the less likely it is transferred into another organism in the lab. Predicting ...Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to predict the lab-of-origin....1 (2018): 4425. PubMed PMID: 30356044. PubMed Central PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher....1 (2018): 3135. PubMed PMID: 30087331. PubMed Central PMCID: PMC6081423.    Additional resources on the...

...Calcium sensors Tracking calcium dynamics that govern such activities as neurotransmitter release is vital...expression, researchers often need to determine these transduction patterns empirically for their own experiments...GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience...research relies on accurate monitoring of neurotransmitters and we are excited to offer AAV for the newest...

...specialized E. coli strain for protein production and gives a quick rundown of some of the strains you can use...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Juchheim in this post. 3. Plasmids 101: E. coli Strains for Protein Production Addgenie Julian Taylor-Parker...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

... different strategies with CRISPR-Cas9. There are so many different variants and strategies out there....Empower and Accelerate Research Discoveries) Award attracted submissions from 99 universities and foundations...desire to understand the early events in the transformation of healthy cells into melanoma cells. They’...diseases at the same time. The limitation of budget restrained us from costly functional analyses.  “We believe...

...build a strategic network to facilitate your company’s development  Don’t be afraid to email strangers asking...likely need a functional prototype to gain any real traction.  This can carry a heavy price tag, especially... to the healthcare landscape.  You will find an extra layer of difficulty launching a company in the health...for internal operations and building external strategic partnerships with universities, companies, hospital...

...just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew it was...candidate, I knew they would look for a candidate with extra experience or a marketing interest that would be...scientists. Tailoring my CV for the job To become an attractive candidate I knew I had to push not only my academic...I had no previous business, marketing or sales training and knew I had to start somewhere. I knew if I...promotions or further education may allow you to transition into various realms of business.  Thank you ...

...biology labs the world over. Scientists have traditionally used Sanger sequencing to validate these constructs...to reconstruct the full individual sequence. Traditional NGS workflows are limited by costly library prep...normalized across a wide range of input DNA concentrations. This process is explained more fully in the...numbers of samples with a wide range of input concentrations (via proprietary chemistry). This turns out...shown in Fig. 2, below, differences in input concentration across a 100-fold range (1ng – 100ng) are normalized...

...setting writing goals, tracking progress, and starting a writing group. Loop Habit Tracker app for Android  ...to get those words out and onto the screen and transform your work into a paper or a grant. Here’s a list...  This app lets you track habits, like writing. It’s basically the equivalent of crossing off days on ...I like to look at these graphs when I need that extra push to stick with my writing routine. The graph...

...ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well as mammalian and human cell...conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently...the Tara Oceans Project datasets. This study demonstrates the power of combining the analysis of publicly...are also thought to play a key role in the transcriptional regulation of many genes. A number of groups...thus far for these latter experiments, such as transcription factors tethered to dCas9, require long time...

...Choosing a lab can be a major decision. A science trainee will spend 4-7 years working for one person and...you’re within a year from the end of your PhD or contract, you should be starting to send out polite emails...important, the end goal during this time is to be trained to think and execute tasks like a scientist. Of...where the topic of your research can make your transition easier – choose a lab that works on problems ...can. You know you are in the zone when you lose track of time because you are engrossed in a task. Things...

...Wen et al., 2018). SRIRACCHA uses the Piggybac transposon to stably integrate a reporter gene that contains..., you’re ready to edit. These cells are then transfected with several different constructs for the reporter... gRNAs by simply changing the gRNA used when transfecting your cells.  A plasmid that contains Cas9. The...plasmid.  With this set up, all successfully transfected cells will express RFP while cells containing... Lastly, once a mutant is identified, the PB transposase can easily and seamlessly remove the integrated...

...brightness When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent...experimental conditions that are related to the imaging strategy and the sample. Practical brightness A protein...developed in our lab uses a reference protein that is translated from the same open-reading frame and separated...ranking of practical brightness can be made. This strategy has been used in yeast by Lee et al (2013) and...

...scientists and the support of their technology transfer offices, Addgene would not be able to have such...to recognize a few scientists who have gone the extra mile for this particular project. John Doench and... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The..., a big thank you to the Addgenies who went the extra mile to create this new service. We knew the scientists...

...selection is a process in which only cells with the trait of interest will survive in the selected growth ...unknown manipulation of the population. This is contrary to reverse screens, where there is removal of ...usually selected. The method you choose to assess traits of interest is highly specific to your scientific...the end of the selection process. Non-functional traits will be automatically eliminated, unlike in screens...Directed evolution of enzymes: Library screening strategies. IUBMB Life, 61: 222-228 (2009). doi: https:/...

...556.7699 (2018): 57. PubMed PMID: 29512652. PubMed Central PMCID: PMC5951633. Lee, Jungjoon K., et al. "Directed....1 (2018): 3048. PubMed PMID: 30082838. PubMed Central PMCID: PMC6078992. Nishimasu, Hiroshi, et al. "...2018): 1259-1262. PubMed PMID: 30166441. PubMed Central PMCID: PMC6368452. Strecker, Jonathan, et al. "...10.1 (2019): 212. PubMed PMID: 30670702. PubMed Central PMCID: PMC6342934. Teng, Fei, et al. "Repurposing... 4.1 (2018): 63. PubMed PMID: 30510770. PubMed Central PMCID: PMC6255809.  Additional resources on the...