... was contributed by Kusumika (Kushi) Mukherjee, editor of Trends in Pharmacological Sciences, a Cell Press...Shinya Yamanaka’s lab, where the reprogramming factors were delivered via retroviral infection [8]. The...Kushi) Mukherjee. Kusumika (Kushi) Mukherjee is the Editor of Trends in Pharmacological Sciences, a Cell Press...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of stem cell research. Best Pract...embryonic and adult fibroblast cultures by defined factors. Cell, 2006. 126(4): p. 663-76. PubMed PMID: 16904174... cells from adult human fibroblasts by defined factors. Cell, 2007. 131(5): p. 861-72. PubMed PMID: 18035408...

...Keep the hood clean Hoods should not be used as storage cabinets. Clutter not only makes it difficult to...air conditioning on) so leave them in a sterile incubator. This also means that any medium or washing buffer...entering the hood and when putting them into the incubator). It also helps to get familiar with the morphology...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...aliquot them into a number of vials, freeze them, and store all but one in liquid nitrogen. The frozen vials...master stock. This ensures that you have many cells stored in their original condition and can replace your...with the Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids...

...development programs for bioscience educators. Using these hands-on tools, educators introduce their students to...This post was contributed by Tim Herman, director of the MSOE center for BioMolecular Modeling and the...dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We created...something tangible. Under the guidance of a trained educator, these instances of focused attention become magical... their labs. Soon after we began working with educators in our Center for BioMolecular Modeling, we spun...our guest blogger Tim Herman! TIm Herman is the director of the MSOE center for BioMolecular Modeling and...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

...guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and a Postdoc...professional development I had a wonderfully supportive mentor and graduate office. I cannot stress how important...my blog helped immensely when CINSO started brainstorming the resources we wanted to provide online. ...in real time! I follow great online science communicators like Phil Plait @BadAstronomer, Emily Graslie...when I was trying to find local informal science educators, and ever since, the State Museum has been the...week. I gained experience explaining science to visitors of all ages, learned a lot about the dinosaurs...Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and a Postdoc...

...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...cloned into a vector containing Cas9 and the PITCh-gRNA. Step 3: Contransfect the PITCh vector with the vector...just upstream of a stop codon. Briefly, the PITCh vector should be constructed with 5’ and 3’ microhomology...PITCh Step 1: Generate microhomologies in the PITCh vector ~20 bp 5’ and 3’ microhomologies are added to ..., and this construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an... vector carrying Cas9 and the PITCh- and locus-specific gRNAs Step 4: Select for puromycin resistant cells...

...conjunction with activator or repressor domains to control gene expression. Base editors can help you make... in a single lentiviral transfer vector or separate transfer vectors. May contain reporter gene (e.g. ...experiment As with any experiment, there are many factors that need to be considered during the planning ... that would work for your system. Addgene has depositor submitted protocols and links to a CRISPR forum...Components of System Application Mammalian expression vector Promoter driving Cas9 expression can be constitutive...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or...

...Science describing the development of “transcriptors,” transistor-like digital genetic switches that enable...Stanford develops engineered DNA systems capable of storing data and computing inside living cells. He's also...cellular computing. These logic gates built from transcriptors are now available at Addgene through the Biobrick...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...the beginnings of computational logic and data storage. What we don't have are the keyboards and displays...mining. There is an absolutely incredible hidden story here. The parts we are releasing simply plug into... plants. We are really plugging into the "stuff sector" if you will, making chemicals and materials, and...

...signaling pathway with many receptors, signaling molecules, transcription factors, and ligands. The presenter... unbiased discovery. With these screens, the investigator generates a cell population where all genes ...contexts. Most of the immune cells we study are migratory, and their exact localization is important for...types of interactions to a large extent involve receptors expressed on the cell surface. Thus, a screen ...targets, preferentially those for which validated inhibitors or even approved drugs already exist. If you ...The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences that you ...strategies related to other nucleases as well as inhibitory and activating approaches.  Many thanks to our...

... for depositing labs to its website, allowing depositors to download their plasmid request data, as a ...information can be of personal interest to the depositor, but it can also be valuable data to share in ...total requests, total countries represented by requestors, year of first reagent sent, year with most reagents...is launched, it will show my data by default. Depositors can download their CSV file from Addgene by following...Dashboard After the upload, the name of the depositor and the date of downloading the CSV is shown (...and the source code is available in the Github repository (you can find the link in the upper right hand...dashboard useful when analyzing and sharing your own depositor metrics!  About the Author Joachim Goedhart is...

...including the internet!) can vary depending on factors like socioeconomic status and geographic location...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...restrictions (or both!) will be a major limiting factor at multiple points. Before you embark on a project... convey enough information to tell a compelling story in an image or do you need a full exhibit? In a ....  Static. Can be difficult to convey an entire story or context via still images. Some things cannot ...information. Primarily useful for data-focused stories. In-person lectures, presentations, etc… Low... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...