We narrowed to 813 results for: cell

... are filled with early mitotic cells as well as necrotic or dead cells (Jiang and White-Cooper, 2003)....interact with each other to generate forces that cause cells and muscles to contract depending on the context...context. Contraction is critical for processes such as cell movement/motility, development, cytokinesis, and...scientists who discovered comr thought the sperm cells in mutant flies looked like a “whole bunch of cookie...are part of a genetic cascade that determines the cell fate of the dorsal mesoderm in developing Drosophila... and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila." Genes...required for oogenesis in Drosophila." The Journal of cell biology 139.7 (1997): 1805-1819. PubMed PMID: 9412474...

...expanded since its adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system...plasmids containing sgRNA sequences into HEK293T cells. The fluorescence intensity was classified into ...is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP plasmid along with...incubation, you assess the level of fluorescence in your cells under a fluorescence microscope. The higher the ...you’ve validated your gRNAs for use in mammalian cells, it’s time to use them in mouse embryos. When researchers...developed as the use of CRISPR/Cas9 in mammalian cells was being explored and it allowed control over each... the mutation will be homozygous for it in every cell; however, if the microinjection was done as the ...

...coordinated by the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation...toxic intermediate buildup as well as controlling cell fate decisions. By using an orthogonal dCas9 protein...for sequence-specific control of gene expression. Cell, 152(5), 1173-1183. (2013). PubMed. Zalatan, J...Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed. Find the Plasmids...crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here: https://www.addgene.org.../article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found here: https://www.addgene.org...or metabolism. Our attempts to manipulate these cellular behaviors often fall short with the generation...

... there is one problem: how to get that into the cells of interest,” Griesbeck said. Because they are chemical...advantage for observing events taking place inside cells that are on the move, such as T lymphocytes. Griesbeck...-binding protein, troponin C (TnC), from muscle cells. They identified a toadfish TnC variant which had...cAMP-responsive element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck...also present challenges in that they are found in cells at much lower concentrations than calcium, requiring...Friedrich et al. Imaging CREB activation in living cells. J Biol Chem. 2010 Jul 23;285(30):23285-95. doi:...understand the connection between molecular- and cellular-level events and behavior. It’s a problem that...

...are commonly found in cells, and without something to block RNase activity upon cell lysis, RNA is degraded...RNases such as RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and...before RNases had a chance to break down RNA upon cell lysis.  In the late 1970’s, Chirgwin and colleagues...keeping your samples cold until homogenization and cell lysis. This can be challenging depending on your...developed ways to “stabilize” RNA within tissues before cell lysis. These products, namely RNAlater®  from Thermo...solutions that work by inhibiting RNase activity within cells or tissues- they don’t actually chemically stabilize...

...expression and needing to work with a transfectable cell line. 2) They require larger expression vectors... of 4 crRNAs in vitro and when expressed in 293 cells. This allows for a single promoter to drive expression... were used.  With transfection, 6.4% of HEK293T cells had edits at 4 of 4 targets when an array of 4 crRNAs...transfected on a single plasmid. This is versus 2.4% of cells transfected with a pool of plasmids that contained...processing of the crRNA array when expressed in cells, but notice how Zetsche et al reverses the orientation...mouse neurons and in vivo. For these experiments, cells were infected with a 1:1 ratio of two AAVs,  one...RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell,163(3), 759-771. PubMed PMID: 26422227. PubMed Central...

...used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG tag...studies (Fujita et al., 2018). In addition to the "in cell" form of this method, in which an engineered DNA-binding...molecule such as the CRISPR complex is expressed in cells to be analyzed, the Fujii lab also developed another...recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed PMID: 26848818. Fujita...‐dC as9 protein for enCh IP analysis." Genes to cells 23.4 (2018): 318-325. PubMed PMID: 29480524. Gilbert...RNA-guided regulation of transcription in eukaryotes." Cell 154.2 (2013): 442-451. PubMed PMID: 23849981. PubMed...of chromatin interactions by biotinylated dCas9." Cell 170.5 (2017): 1028-1043. PubMed PMID: 28841410. ...

...if the cell dies from injury, this protein leaks out into the cytoplasm, migrates out of the cell, and ...Keytruda) causes T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab...cause inflammation, which can cause mesothelial cells to mutate and ultimately form cancerous tumors.  ...resection), systemic chemotherapy to kill cancerous cells that may have spread beyond the point of origin,...successful in killing many (if not all) of the cancer cells that may be left behind after surgery, while being...and binds cells that can begin an immune response. This important biomarker can be used to distinguish between...

...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage)....extensively to screen for molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA...field of eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully...The reporter gene then allows detection of those cells where binding between the proteins of interest has...alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If the problem is that your proteins are not post-translationally... DNA and activate a reporter gene in eukaryotic cells, fusion proteins must gain access to the nucleus... the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729-736. PubMed PMID: 3907859. 4. Fields...

...epigenome editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant...tissue is identifying the cells you are studying. Antibodies can help reveal cellular identities by targeting...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain... will only be transiently present in transduced cells, limiting off-target editing. Cargo size limits ...site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime ... Recombinant antibodies for marking cell types in the nervous system By Ashley Waldron The mammalian nervous... nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who...