We narrowed to 969 results for: ARC;

...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...

...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...

...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...McDade for creating the Expression Systems table. Marcy Patrick contributed to the writing of this post....

...for gene knockout, activation, repression, and barcoding.  3) Are you trying to target every gene in the...thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing CRISPR libraries...

...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...

...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

...the Future Initiative. Endy's team made news in March with a publication in Science describing the development...translation initiation." Nature Methods. 10, 354-360 (10 March 2013). ...

...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...

...nm peak is more specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator...Qiagen Gene Expression Newsletter. Issue 15/10. March 2010. https://www.qiagen.com/de/~/media/1ea8aec3bfa24543a28fcaea25986514...

...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...

...Alternative envelopes are powerful tools as they allow researchers to target transduction to a particular cell type...be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing...

...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

...A recent survey of PhDs found that many researchers feel they lack formal training in a variety of transferable...'re helping to fill this gap by both helping researchers recognize their MANY transferable skills learned...

...pathogen more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein...Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org...

... children and patients with skin disorders for research and clinical trials, as it allows access to a ... fellow at Massachusetts General Hospital with research interests in the fields of gene editing and stem...