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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 838 results for: ANG

Showing: 741 - 760 of 838 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where TU is transducing units... This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the master ...directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently pipetting the... of the well and tilt the pipette tips at a 45° angle. Count to 20 while slowly and gently dispensing ...Table: Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral...

  2. Common Injection Routes in Mice

    Type
    Blog Post
    ...behind the eye. IV injections are used for a broad range of substances, as it circulate what is injected ...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...technology, plant cells have been used to express a wide range of proteins including antibodies and pharmaceuticals...

  14. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... or Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt ends...amount of DNA in the reaction, incubation time can range from 45 mins to overnight. For diagnostic digests...compatible sticky ends, you will need to fill in the overhangs and conduct a blunt end ligation. Use T4 DNA Polymerase...

  15. MXS Chaining

    Type
    Blog Post
    ...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...

  20. An Introduction to Adenovirus

    Type
    Blog Post
    ... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs...
Showing: 741 - 760 of 838 results