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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 835 results for: ANG

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Showing: 741 - 760 of 835 results

  1. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... Nonetheless, we recommend performing a buffer exchange before using the purified AAV in vivo . The iodixanol... Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration Note: Both steps could be completed...Pool clean fractions. Concentration and buffer exchange: Stocks of Poloxamer 188 Poloxamer 188, 10% solution...adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...enzyme, or enzymes that have compatible overhangs or no overhangs after digestion, you will need to use ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

  3. Common Injection Routes in Mice

    Type
    Blog Post
    ...behind the eye. IV injections are used for a broad range of substances, as it circulate what is injected ...

  8. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...technology, plant cells have been used to express a wide range of proteins including antibodies and pharmaceuticals...

  15. AAV ddPCR Titration

    Type
    Protocol
    ...outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where GC is genome copies ...slowly to avoid generating aerosols. Be sure to change tips each time. Dilution 1 (20X): 5 µL in 95 µL...directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently aspirating ... of the well and tilt the pipette tips at a 45° angle. Count to 20 while slowly and gently dispensing ...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    ... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs...

  20. MXS Chaining

    Type
    Blog Post
    ...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic...
Showing: 741 - 760 of 835 results