...on the marketing or business background of the applicant. However, although this position did look for ... interviews Studying for interviews is really important. For my Postdoc position, I tried to read at least... current sales position interview was just as important. The problem was I knew I had a knowledge gap....teaches you about selling products which is an important part of the business and allows you to work with...

...enable? Boyden: There are many examples where you want to activate two sets of neurons and see how different...quarter of a million people have some kind of neural implant already. If we can use light to more specifically...many of them very popular. Why has it been so important to you to distribute these tools as freely as ... There are lots of neuroscientists out there; we want them to use these tools, so we can focus on the ...

...of a cell - cell free extracts Typically, if you want to make a protein – say green fluorescent protein...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and... hydrogels that they can touch. With BioBits, we want students to make the connection between the facts...Learn how the Arabidopsis research center brings plant science to life Resources on Addgene.org Browse...

...locked down, a South African company wanted to test if a type of antibodies from an Andean mammal called alpaca...diseases, so we were left with no choice but to quarantine in our houses, feeling helpless about the situation... more efficient, open, and collaborative. We now want to use this potential to make a difference not just...

...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,...our all-company meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting...Meetings are for talking, not reading: Send around relevant documents, like proposals to review or project...discussions can happen “off-line” with just one or two relevant people. How often do you need to meet? Use trial...

...have that you really want, please let us know! And if you study chromatin and want to start sharing your...daily basis, the lists below provide a snapshot of relevant resources and a good jumping off point. The following...that histone genes frequently have many different variant names, not all of which can be listed here. If ...

...commonly a plasmid and can infer genetic advantages such as antibiotic resistance. Bacterial conjugation...primary mechanism by which bacteria have gained antibiotic resistance and virulence. Scientists have studied...integration site. The integrated phage remains dormant until it is triggered to enter the lytic cycle....

...enlarge) As we look ahead to the things to come, we wanted to take a look back at some of the contributions...expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting... from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope expressing...Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n...

...including hormones, neurotransmitters, photons or odorants, to elicit downstream signaling cascades.  So ... cell membrane. Heterotrimeric G proteins are important molecular switches in signal transduction pathways...in peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek ...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

...into my role, I realized that actually, I didn’t want to go to grad school. But, I didn’t know what else...great fit for my skillset and interests. As such, I want to be able to share more about this nontraditional...order to keep everything running smoothly, you’ll want to be really organized. Often, product managers ...behind a common vision. Use your network to your advantage  As the profession gains more traction in the...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...expression in the liver after systemic delivery.  Importantly, this is true in both mice and nonhuman primates...side effects, by allowing the user to select for variants that infect the CNS while selecting against those... knock-in long sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally...

... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem? A: Re-administration...for AAV due to the development of neutralizing antibodies in animal models. All of our engineered AAV are...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions.    ...

...you're trapped in a pattern of small errors that significantly impacts your experiments. What do you do when... a failed experiment.  For example, I used to constantly underestimate time needed to prep for an experiment...During really stressful days, I would even take advantage of incubation periods to have another short "quiet...maybe especially) suggestions you are initially resistant to. Just like any other experimental approach,...

...and indicate why other neuroscience newbies might want to get into the field. Find Optogenetics Plasmids...There were, of course, many companies selling antibodies, microscopes, and dissection tools, but there...FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if you’ve got a ...neuroscientist looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene...

...students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you tell...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...preparing local (Massachusetts), economically disadvantaged students for paid summer internships. A summer...Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused on education and education...

...Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering...process involve repairing chromosomal DSBs. It is important to know that cells possess two major repair pathways... and how these pathways work, as this could be relevant when planning your experiment. Here, we’ll discuss...Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR...

...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can...they have to give you theirs! Think about how you want to introduce yourself and what you do. You can probably...night poker games at Gordon Conferences). You don’t want to be so exhausted that you can’t get a lot from...

...doing interesting and relevant work in your field! However, there’s no guarantee you’ll get a response...to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...always faster to order from us!) Ask the PI If you want a plasmid for free, you can always reach out to ...

...in my more recent positions outside of the lab. Granted, I currently work on the Scientific Support Team...thesis as it is for helping your team reach an important milestone at work.   While I was in graduate school...work through it. Ask yourself if you missed any important details. Or challenge yourself to think of more...that this skillset is a part of your toolkit is important. Paired with your other skills, both technical...

...newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR technology...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective... the cellular MMR machinery is disrupted via a dominant negative mutation in MLH1 (PE4 = PE2+MLH1dn and...improve gene editing performance at therapeutically relevant gene targets and expand the application to the...