We narrowed to 958 results for: ARC
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TypeBlog Post...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...McDade for creating the Expression Systems table. Marcy Patrick contributed to the writing of this post....
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Genome-wide Screening Using CRISPR
TypeBlog Post...for gene knockout, activation, repression, and barcoding. 3) Are you trying to target every gene in the...thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing CRISPR libraries... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...the Future Initiative. Endy's team made news in March with a publication in Science describing the development...translation initiation." Nature Methods. 10, 354-360 (10 March 2013). ... -
MXS Chaining
TypeBlog Post...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...but I also tried not to be an alarmist. The overarching guideline for all of these questions, and for... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...nm peak is more specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator...Qiagen Gene Expression Newsletter. Issue 15/10. March 2010. https://www.qiagen.com/de/~/media/1ea8aec3bfa24543a28fcaea25986514... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...Alternative envelopes are powerful tools as they allow researchers to target transduction to a particular cell type...be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in bacteriology and... Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research microbiologist specializing in bacteriology and... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... children and patients with skin disorders for research and clinical trials, as it allows access to a ... fellow at Massachusetts General Hospital with research interests in the fields of gene editing and stem... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post... 16 labs around the world and can be used by researchers to validate their qPCR assay (Lock et al., 2010...titrating AAV vectors." Medical science monitor basic research19 (2013): 187. PubMed PMID: 23828206. PubMed Central... -
Lab to Office Culture Shock
TypeBlog Post...→ office culture shock? Working in an academic research lab involves a few key features that can be difficult...community (and sometimes conduct their own independent research!). There are also organizations like Science from... -
Hot Plasmids: Fall 2024
TypeBlog Post...pathways and spontaneous mutations. Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...target!) and use them in numerous fields including research and development, diagnostics, drug discovery, ...interfere with conjugates. Make sure to do your research before conjugating at the bench. Indirect ELISAs... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus spp., Thiobacillus...enteritidis, S. typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas ... -
Bioinformatics at Addgene
TypeBlog Post...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language. The modern architecture of Nextflow included built-in support for containers... -
Transferable Skills Guide: Creativity
TypeBlog Post...A recent survey of PhDs found that many researchers feel they lack formal training in a variety of transferable...'re helping to fill this gap by both helping researchers recognize their MANY transferable skills learned... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...pathogen more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein...Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org... -
Career Insights: Technical Support Specialist
TypeBlog Post...work for biotechnology companies and help other researchers use the company’s products successfully. In technical...involve interacting with other departments such as research and manufacturing, quality assurance, product ...