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Showing: 741 - 760 of 952 results

  1. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

  2. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

  3. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

  4. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...

  6. Genome-wide Screening Using CRISPR

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    Blog Post
    ...for gene knockout, activation, repression, and barcoding.  3) Are you trying to target every gene in the...thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing CRISPR libraries...

  7. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...

  8. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

  10. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post
    ...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...

  11. MXS Chaining

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    Blog Post
    ...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...

  12. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...Alternative envelopes are powerful tools as they allow researchers to target transduction to a particular cell type...be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing...

  13. Lab to Office Culture Shock

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    Blog Post
    ...→ office culture shock? Working in an academic research lab involves a few key features that can be difficult...community (and sometimes conduct their own independent research!). There are also organizations like Science from...

  14. Bioinformatics at Addgene

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    Blog Post
    ...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  15. Transferable Skills Guide: Creativity

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    Blog Post
    ...A recent survey of PhDs found that many researchers feel they lack formal training in a variety of transferable...'re helping to fill this gap by both helping researchers recognize their MANY transferable skills learned...

  16. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...pathogen more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein...Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12:1016 . https://doi.org...

  17. Hot Plasmids: Fall 2024

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    Blog Post
    ...pathways and spontaneous mutations.  Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant...

  18. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post
    ...target!) and use them in numerous fields including research and development, diagnostics, drug discovery, ...interfere with conjugates. Make sure to do your research before conjugating at the bench.   Indirect ELISAs...

  19. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus spp., Thiobacillus...enteritidis, S. typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas ...
Showing: 741 - 760 of 952 results