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  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...a cell culture model. What we still don't know about Cpf1 In their original paper characterizing Cpf1...Addgene Blog Catch Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...additional helper sequences (2-3). As alluded to above, termination and polyadenylation of Polymerase II...how the Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids ...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A.,...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

  5. Common Injection Routes in Mice

    Type
    Blog Post
    ...Routes of administration. In H. Hedrich (Ed.), The Laboratory Mouse. Academic Press. Turner, P. V., Brabb, ...M. A. (2011). Administration of substances to laboratory animals: routes of administration and factors...

  6. Pooled Library Amplifications

    Type
    Blog Post
    ...First, you’ll need to collect specific information about your library: the size of your library (How many...with Addgene. If you have any specific questions about designing your library amplification protocol, feel...

  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific... Listen to Our Interview with Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators...

  12. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Resources on the Addgene Blog Learn about inducible promoters Learn about repressible promoters Check out ...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield ...Topic Page Learn How to Verify Your Plasmid Learn about Common Lab E. coli Strains Additional Resources...

  15. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

  17. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... work. After all, a stick-figure internet comic about math probably didn't sound like a great idea to ...  Selecting the method Once you’ve answered the above questions, you can create your short list of options...

  18. MXS Chaining

    Type
    Blog Post
    ...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...
Showing: 741 - 760 of 963 results