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We narrowed to 969 results for: Arc

Showing: 741 - 760 of 969 results
  1. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    ...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...
  2. Transferable Skills Guide: Managing a Team

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    Blog Post
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...
  3. Truncated gRNAs for Regulating Gene Expression

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    Blog Post
    ...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...
  4. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...
  5. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers... Addgene.org Find mammalian plasmids for your research Find viral vectors for infecting mammalian cells...
  6. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...
  7. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...
  8. Genome-wide Screening Using CRISPR

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    Blog Post
    ...for gene knockout, activation, repression, and barcoding.  3) Are you trying to target every gene in the...thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing CRISPR libraries...
  9. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...
  10. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...
  11. MXS Chaining

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    Blog Post
    ...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...
  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...
  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Alternative envelopes are powerful tools as they allow researchers to target transduction to a particular cell type...be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing...
  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...pathways and spontaneous mutations.  Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant...
  15. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post
    ...target!) and use them in numerous fields including research and development, diagnostics, drug discovery, ...interfere with conjugates. Make sure to do your research before conjugating at the bench.   Indirect ELISAs...
  16. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus spp., Thiobacillus...enteritidis, S. typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas ...
  17. Bioinformatics at Addgene

    Type
    Blog Post
    ...importance of bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...
Showing: 741 - 760 of 969 results