We narrowed to 972 results for: Arc

...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

...from one serotype and the capsid of another. Researchers have used these psuedotypes to further improve...-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S...

...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...

...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...

...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...

...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...McDade for creating the Expression Systems table. Marcy Patrick contributed to the writing of this post....

...for gene knockout, activation, repression, and barcoding.  3) Are you trying to target every gene in the...thousands of gRNA plasmids, discerned only by a unique barcode on each plasmid. As such, sequencing CRISPR libraries...

...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...

...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

...the Future Initiative. Endy's team made news in March with a publication in Science describing the development...translation initiation." Nature Methods. 10, 354-360 (10 March 2013). ...

...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...but I also tried not to be an alarmist.  The overarching guideline for all of these questions, and for...

...nm peak is more specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator...Qiagen Gene Expression Newsletter. Issue 15/10. March 2010. https://www.qiagen.com/de/~/media/1ea8aec3bfa24543a28fcaea25986514...

...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...

...Alternative envelopes are powerful tools as they allow researchers to target transduction to a particular cell type...be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing...

...technologies have the potential to transform neuroscience research by bringing the precision of molecular targeting...

...→ office culture shock? Working in an academic research lab involves a few key features that can be difficult...community (and sometimes conduct their own independent research!). There are also organizations like Science from...