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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 14 results for: Arc

Showing: 1 - 14 of 14 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest as a loud...single sample. They are often used for screening, barcoding, or other high throughput multiplexed experiments...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...see these vectors, visit Addgene’s website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos...example, the Whitehead Institute for Biomedical Research hosts an siRNA Selection Program that can be accessed...Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences that match the pattern AA(N ...N 19 ). If no suitable match is found, search for NAR(N 17 )YNN, where N is any nucleotide, R is a purine...

  3. Ligation Independent Cloning

    Type
    Protocol
    ...-Bsa4 as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors...transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning vectors...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    .... From a single copy of DNA (the template), a researcher can create thousands of identical copies using...synthesizes and ships them as a lyophilized powder. The researcher then needs to reconstitute their primers in liquid...

  8. Video Library

    Type
    Protocol
    ...Welch to discuss her career path, differences in research culture between the US and Australia, and advice...

  9. Using a Light Microscope Protocol

    Type
    Protocol
    ...Introduction Microscopes are emblematic of biological research and are found in many different types of laboratories...

  11. Pouring LB Agar Plates

    Type
    Protocol
    ...antibiotic resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid...

  14. Kit Free RNA Extraction

    Type
    Protocol
    ...mM Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol®...
Showing: 1 - 14 of 14 results