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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 962 results for: Cre

Showing: 741 - 760 of 962 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

  6. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  8. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  10. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...your team you will have a much better chance of creating a high-performing team - which is every manager...! Remember that delegating is not just a way to create more time for yourself, but it will help develop...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Pre-made viruses have become increasingly accessible and are useful for saving time and avoiding potentially... collected over the course of a couple days to increase yield. The collection process is a cycle of collecting...

  12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and...have a kit!). Choosing a degron tag Are all tags created equal? No! Choosing the ‘right’ tag does depend...

  17. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...

  18. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...This amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence...detected via a fluorescent signal. The signal increases proportionally to the amount of product produced...

  19. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...learning from the careers of others were key to creating my career path. Luckily, in grad school much of...

  20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...gRNA.  While this methodology has proven to be incredibly valuable to scientists, there are some potential...Harrington, Lucas B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1...
Showing: 741 - 760 of 962 results