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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 967 results for: Cre

Showing: 741 - 760 of 967 results

  1. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...synthesized at the ER, delivered to the vacuole via the secretory pathway, and then cleaved to generate an active...In both cases, red fluorescence in the vacuole increased with longer exposure to autophagy-inducing conditions...

  2. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Most coaches have websites you can review and most create useful online content in the form of blogs or articles...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  3. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength...for funding improves the associated skill sets. Increased experience in communicating and motivating research...

  4. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

  10. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

  12. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  14. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Pre-made viruses have become increasingly accessible and are useful for saving time and avoiding potentially... collected over the course of a couple days to increase yield. The collection process is a cycle of collecting...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and...have a kit!). Choosing a degron tag Are all tags created equal? No! Choosing the ‘right’ tag does depend...

  16. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...

  17. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...This amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence...detected via a fluorescent signal. The signal increases proportionally to the amount of product produced...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...
Showing: 741 - 760 of 967 results