We narrowed to 809 results for: Eng

1. Plasmids 101: Optimizing Plasmid Yields

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   ...of endA+ strains Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...

2. Experimenting with New Careers while in Grad School

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   ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

3. Genome-wide Screening Using CRISPR

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   ...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...

4. Light Sheet Fluorescence Microscopy

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   ...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

5. Tips for Using BLAST to Verify Plasmids

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   ...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

6. Simplify Cloning with in vivo Assembly

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   ...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...

7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

8. Getting to Know Expansion Microscopy

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   ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...

10. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...

11. Plasmids 101: Colony PCR

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    ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...

12. A Career in Grants: How To Become a Grant Professional

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    ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

13. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

14. Negotiating Work and Life: How to Find the Joy

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    ...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...

15. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

16. New Tools Enable CRISPRa for Neuroscience Applications

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    ...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to...

17. Plasmids 101: Codon usage bias

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    ...‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli." ...

18. Transferable Skills Guide: External Collaborations

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    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...

19. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...describing the labeled regions, as well as the strength and density of labeling in each. This allows users...

20. Twenty Years of Sharing: Expanding Accessibility

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    ...countries means that our shipping team is always engaged in some form of problem-solving. When universal...