We narrowed to 802 results for: Eng

1. Getting to Know Expansion Microscopy

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   ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

2. Simplify Cloning with in vivo Assembly

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   ...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...

3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...

5. Plasmids 101: Colony PCR

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   ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...

6. A Career in Grants: How To Become a Grant Professional

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   ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

7. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...that provides a community for biologists and bioengineers to share their educational materials, protocols...

8. Your Lentiviral Plasmid FAQs Answered

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   ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

9. Negotiating Work and Life: How to Find the Joy

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   ...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...

10. Transferable Skills Guide: External Collaborations

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    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...

11. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...Induced Pluripotent Stem Cells (iPSCs): Benefits, Challenges and the Road Ahead." Journal of Alzheimer's disease...

12. AAV Titers: Where Do They Come From And What Do They Mean?

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    ...28546538. PubMed Central PMCID: PMC5445070. 6. Wang, Feng, et al. "A reliable and feasible qPCR strategy for...

13. Transferable Skills Guide: Career Planning Resources

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    ...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    .... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...

15. How to Write a Scientific Review Article

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    ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

16. Mesothelioma - Causes, Symptoms, and Treatment

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    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...

17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

18. New Tools Enable CRISPRa for Neuroscience Applications

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    ...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to...

19. To Codon Optimize or Not: That is the Question

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    ...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...

20. Plasmids 101: Plasmid Incompatibility

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    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from...