We narrowed to 805 results for: Eng

1. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   ...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

2. Tips for Using BLAST to Verify Plasmids

   Type

   Blog Post

   ...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

4. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   ...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...

5. Getting to Know Expansion Microscopy

   Type

   Blog Post

   ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   ...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...

7. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   ...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...

8. Plasmids 101: Colony PCR

   Type

   Blog Post

   ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...

9. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

10. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

11. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    ...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...

12. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

13. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to...

14. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    ...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...

15. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from...

16. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ...‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli." ...

17. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...

18. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    ...countries means that our shipping team is always engaged in some form of problem-solving. When universal...

19. Hot Plasmids: Fall 2024

    Type

    Blog Post

    ...common microscopy methods. Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence...

20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    .... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...