We narrowed to 1,028 results for: LEA
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TypeBlog Post...ever like to write about a recent plasmid deposit please sign up here. Human whole genome sgRNA iBAR ...calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase that...
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Meet the New Addgene Board Members!
TypeBlog Post...Association and it was through this connection that she learned more about Addgene. “The mission to share critical...and Ear and Harvard Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has... -
RUBY-Red Siliques
TypeBlog Post...segregating mutation. Selecting these plants is a learned skill that takes practice and is difficult for ...enzymes expressed under the same promoter with self-cleaving 2A site linkages and a terminator. Image from ... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog Post...sometimes up to millions of new copies, which are released from the cell like baby spiders from an egg sac...our Viral Vectors 101 eBook, out if you want to learn more. References and resources References Balloux... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...against the variants? Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard...Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources on Addgene.org... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here. Optogenetic plasmids...00787-6 Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...and running miLab to describe some key lessons I learned while becoming a health science entrepreneur at...First impressions are everything and you want to leave people remembering your potential as an entrepreneur... -
Celebrate National Book Month with Addgene
TypeBlog Post...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...Plasmids 101 Topic Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post... quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince... -
Addgene Depositors Get More Citations
TypeBlog Post... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...questionnaire to the participants to learn about what they were looking to learn in the workshop so they could... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...said. “We also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city... -
K. phaffii: Rising to the Occasion
TypeBlog Post...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...