We narrowed to 1,018 results for: LEA
-
TypeBlog Post...questionnaire to the participants to learn about what they were looking to learn in the workshop so they could...
-
Celebrate National Book Month with Addgene
TypeBlog Post...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post... quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...said. “We also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog Post...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city... -
K. phaffii: Rising to the Occasion
TypeBlog Post...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ... -
Addgene Depositors Get More Citations
TypeBlog Post... over 6,500 requests for these plasmids. While clearly an outlier, this story had us wondering: is there...journals gets more citations and is more likely to lead to an Addgene deposit, since scientists may believe... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold... -
Finding and Joining Your Dream Lab
TypeBlog Post... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...critical roles in nerve and muscle function, hormone release, and cell signaling throughout the body. Because...Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR). ... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post...scientists? Curiosity? Passion? A love of learning? No. Or at least not entirely. What truly unites us all... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...blog posts on base editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...consider them worth the trouble. Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse...potential collaborators and interested audiences. Please visit our team wiki or contact us at igemiiserb... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research Learn about our viral service ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...Symposium on Cancer, Inflammation and Immunity and learn more about “... recent findings in basic immunology... autophagy can be found here. Fun fact: I also learned that Professor Kroemer often functions as one of... -
How Addgene Creates Content
TypeBlog Post...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style. ...