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Showing: 741 - 760 of 1011 results

  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...following decades, Phillips made the decision to release the K. phaffii expression system to the general...phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering. A ...

  2. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ...

  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both...consider for future work placements. The chance to learn and collaborate all while experiencing a new city...

  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

  6. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  7. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert with Fluorecent...Post-translational Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible ...

  8. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...

  9. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

  10. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    ...Cas9n (a D10A nickase mutant) to nick, rather than cleave DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(...CRISPR collection Find plasmids for your research  Learn about our viral service ...

  14. AAV Q&A with Tim Miles

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    Blog Post
    ... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...for very high MOI on top of very high coverage. Please see this paper for more information about PHP.eB...

  16. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  18. How Addgene Creates Content

    Type
    Blog Post
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...

  19. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...
Showing: 741 - 760 of 1011 results