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We narrowed to 1,015 results for: LEA

Showing: 741 - 760 of 1015 results

  1. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...better. AAV transport and transgene expression It’s clear that we need high titers, as contact between the...the cell interior, causing less particles to be released into suspension and lowering the number of viral...

  4. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Biochemistry or International Finance. A book club could lead to smart, sometimes contentious, discussions. I ...comments! Additional resources on the Addgene blog Learn about company culture at Addgene Read about other...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools... on the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR...

  6. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...he never eats “anything with chemicals in it,” leaving me to wonder how he has survived this long without...to understand what people know – or, at the very least, believe – to be true so that we can tailor our ...

  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult...said. “We also really need to get a handle on how cleavage sites are repaired.” Those insights will be critical...

  9. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter activity persisted...with other pathways. They also reduced system leakiness and increased dynamic range to around 100-fold...

  10. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ... is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In Concert with Fluorecent...Post-translational Modification Not Required Required Photobleaching/Phototoxicity Not susceptible Susceptible ...

  11. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... convince the PI to choose me?” We asked three leading professors how they hire scientists for their labs... Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments Thank you to Dr...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

  13. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ... canonical 20 amino acids. But in your quest to learn more about your protein of interest, you may find... both translation and fidelity of translation, leading to system-wide effects. And on top of that you ...

  14. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...retrograde AAV work are not yet understood, one thing is clear: this application opens a lot of doors in neuroscience...Resources on the Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's...

  15. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation of ...example of how sharing of data and molecular tools can lead to faster and more efficient science. Researchers...

  16. How Addgene Creates Content

    Type
    Blog Post
    ...involved yourself. You probably won’t be surprised to learn that Addgene’s mission statement extends beyond ...and our graphic designer to make sure things are clear, accurate, and in line with Addgene style.    ...
Showing: 741 - 760 of 1015 results