We narrowed to 809 results for: Mak

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   ...target cells (Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery...

2. Hot Plasmids: Winter 2025

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   Blog Post

   ...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...

3. Common Injection Routes in Mice

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   ...that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...

4. Viral Vectors 101: Producing Your rAAV

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   ...of rAAV production.  Of course, you will want to make sure that your final stocks only contain rAAV. To...

5. Antibodies 101: Flow Compensation

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   ...will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ...

6. Plasmids 101: Degron Tags

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   Blog Post

   ...degron will behave with a new protein, but you can make an informed choice! References and Resources References...

7. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

8. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...is required for NgAgo optimization before we can make any major conclusions: That said, many people are...

9. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   Blog Post

   ...ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...

10. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ... Using this technique, researchers have begun to make important discoveries in various biological fields...

11. Simplify Cloning with in vivo Assembly

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    ...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

12. AAVs in Retinal Gene Therapy

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    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

13. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...

14. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

15. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...added log-normal noise to each of the terms which make up our distribution, resulting in the following ...

16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...decide which ones to use? Here are some tips for making those decisions: Always always always look at ...

17. An Introduction to Adenovirus

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    Blog Post

    ...process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating...

18. Getting to Know Expansion Microscopy

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    ...synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the...

19. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...number of administrative and practical decisions to make. How often will you hold it? How many people do ...

20. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ... DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D fractal...