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We narrowed to 940 results for: Mpl;

Showing: 741 - 760 of 940 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

  2. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... Reddit AMA Perhaps we should start with some examples of a bad panel discussion? You know, the one where... audience members a chance to participate. For example, at a recent event on nonprofit science organizations...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  5. Common Injection Routes in Mice

    Type
    Blog Post
    ...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

  10. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process...postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In academia...

  11. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...attention on social media. I gained a concrete example to use to promote future attempts and learned a...roles of scientists outside of academia One recent example: Don't call me a dropout: Why science needs more...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...low RNA editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...

  16. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

  19. How to Be an Excellent Trainee

    Type
    Blog Post
    ...experience In research there is a saying that if the sample prep is poor, then the data will be poor. The same...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...
Showing: 741 - 760 of 940 results