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  1. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...s41586-021-03886-5 Additional resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for ...beyond spCas9 Cas proteins More resources on the Addgene Website CRISPR Plasmids and Resources CRISPR...
  2. Plasmids 101: FLEx Vectors

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    Blog Post
    ...PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao...Wickersham Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering...
  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post
    ...are publicly available, including those found at Addgene! Figure 1: Strategy for assembling the barcoded...PubMed Central PMCID: PMC4780610. Resources on the Addgene Blog Learn Best Practices for Designing Your gRNA...about CRISPR/Optogentics Tools Resources on Addgene.org Catch up on Your CRISPR Background with Our ...
  4. Tips for Titering Your Lentiviral Preps

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    Blog Post
    ...Additional Resources on Addgene.org Learn How We Do Viral Production at Addgene Read Our Lentiviral Guide... PMCID: PMC514534. Additional Resources on the Addgene Blog Read Our Lentivirus FAQ Learn How Lentivirus...
  5. The Future of Research Symposium Boston 2015

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    Blog Post
    ...biomedical_research_wgreport.pdf Resources at Addgene Learn More about Careers in Science in Our Career...Advice on Networking at Scientific Meetings from Addgene Executive Director Joanne Kamens Learn how to be...
  6. Important Considerations When Using AAVs

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    Blog Post
    ...tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location, location, location! What do buying...populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors...PubMed PMID: 28073291. Additional Resources on the Addgene Blog AAV: A Versatile Viral Tool for Gene Expression..., AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue...Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use...
  7. A Guide to Getting Started in Undergrad Research

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    Blog Post
    ...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career...science.caredit.abi6939 Additional resources on the Addgene blog Check out our resources for early career ...
  8. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...different research applications they can be used for. Addgene’s Antibody Plasmid Collection includes plasmids ...10.3389/fcimb.2013.00072 Additional resources on the Addgene blog: Read about another reagent sharing platform...
  9. Custom CRISPR Screens & the Green Listed Software

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    Blog Post
    ...available through Addgene. This means you could perform a screen with one library from Addgene, and then use...PMCID: PMC5292764. Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design ...melanogaster, and T. gondii are available through www.addgene.org/crispr/libraries/. Additional libraries are...gRNA Tips for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening...
  10. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post
    ...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...
  11. 6 Tips for Grant Writing

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    Blog Post
    ... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...
  12. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post
    ...be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning...PMCID: PMC3207894.  Additional Resources on the Addgene Blog Read other Plasmids 101 blog posts New to...Download the Plasmids 101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find...
  13. dTAG - You're it!

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    Blog Post
    ...generated a series of plasmids that are available from Addgene. These plasmids enable lentiviral expression of...molecule: dTAG-47 Additional Resources on the Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional...endogenous gene tagging in human cells Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for...
  14. Plasmids 101: Protein Expression

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    Blog Post
    ... use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University...proteins in plant root exudates Resources on the Addgene Blog Learn about inducible promoters Learn about...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...
  15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post
    ...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...
  16. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post
    ...AAV plasmids containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice... other tissues.  Additional resources on the Addgene blog Read more about using AAV for retinal gene...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...
  17. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post
    ...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...
  18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post
    ...that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP...References and resources:  Additional Resources on the Addgene blog CRISPR101: Editing the Epigenome  Mapping...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...
Showing: 741 - 760 of 1077 results