We narrowed to 1,091 results for: addgene
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TypeBlog Post...PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology blog posts Read about synthetic...Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...of our Plasmids 101 blog posts Resources on Addgene.org Check out our microbiology resource page Deposit...
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FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich KD et al, Nat Chem Biol 2018. PubMed...pUC-L1L4 and pUC-L3L2. Find the 2in1 plasmid kits at Addgene! Mehlhorn DG et al, Methods Mol Biol, 2018. PubMed... Various Addgenies... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics:.../help/spectral-overlap/ More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies... -
Antibodies 101: Secondary Antibodies
TypeBlog Post... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene .... References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence... -
Antibodies 101: Isotypes
TypeBlog Post...an Antibody animation Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...www.ncbi.nlm.nih.gov/books/NBK27162/ Additional resources on the Addgene blog Learn about the advantages and disadvantages... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...mVenus as a reference protein is available from Addgene. Another way to dodge cellular variation observed...based biosensors. Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post... Click Here to Find Viral Vector Resources at Addgene! References and Resources 1. Roe T, et al. “Integration...PMCID: PMC1368960. Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie...Lentiviral Transduction Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral... -
Visualizing Protein Turnover In Situ
TypeBlog Post...PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent microscopy techniques - Which...protein-protein interactions Deposit plasmids with Addgene! ... tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids Use FRET to study... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection ...10.1101/pdb.top099101 Additional resources on the Addgene blog Read about another common type of antibody... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...editing rate of ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted.../s41587-020-0453-z Additional resources on the Addgene blog Browse our blog posts on base editing Read... in our CRISPR 101 blog series Resources on Addgene.org Find base editors for your research Read our... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...additional pooled CRISPR libraries, also available at Addgene, enabling systematic investigation of the cellular...the use of pooled libraries in your experiments, Addgene recommends that you review the steps for how to... -
Hot Plasmids: Summer 2024
TypeBlog Post...TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR” version...popular target for labeling these cells. Check out Addgene’s Neuroscience Antibody Collection to find a recombinant...patterns from recombinant antibodies produced at Addgene (N206A/8R) were compared to the hybridoma-derived... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist ...find validated gRNAs for your next experiment in Addgene's Validated gRNA Sequence Datatable. A more extended...://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen to our Podcast with John Doench Learn... the Broad Institute and has worked with many Addgenies to help improve the understanding, curation, and...Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR Basics with Our CRISPR... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...Resources on Addgene.org: Check out the Addgene AAV guide Find ready-to-use AAV preps from Addgene's viral ...10.1093/hmg/ddv386 Additional resources on the Addgene blog: Find tips for a first time AAV user Learn... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...Addgene is proud to announce that we recently acquired the ability to distribute plasmids with the piggyBac... to help us expand this useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase...PMCID: PMC3627465. Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase...Repair Is Used for Genome Editing Resources on Addgene.org Find Vectors with the piggyBac™ Transposon Catch... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use...CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage...CRISPR system in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 ...://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ... out about CRISPR Multiplexing Resources on Addgene.org Browse our CRISPR Guide Pages Find Bacterial... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog Post...appreciate the humor in science and in the lab? Addgene is after all a place that encourages people to ... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858. Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...optogenetics Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors.../s41592-019-0583-8 Additional resources on the Addgene blog Read more about the PHP.eB AAV capsid Read...