We narrowed to 841 results for: ang
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TypeBlog Post...probably difficult for you to ascertain where on this range your question falls. Here are some things to keep...
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Plasmids 101: FLEx Vectors
TypeBlog Post...Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. PubMed PMID: 18614669. PubMed Central... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...A) signals like BGH. References Peters JM, Vangeloff AD, Landick R. Bacterial Transcription Terminators... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...becomes obsolete amazingly fast, but with a wide range of skills among our members we’ve managed to learn... -
Plasmids 101: Protein Expression
TypeBlog Post...technology, plant cells have been used to express a wide range of proteins including antibodies and pharmaceuticals... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...wanted to see how low we could go. We prepared a range of spiked samples from 0.1-20% and discovered that... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...editing efficiency such as the length of the RTT overhang, the GC content of the primer binding site, and... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog Post...the markers in the panel. This complexity index ranges from 0-1, with a value of “0” indicating those ... -
Common Injection Routes in Mice
TypeBlog Post...behind the eye. IV injections are used for a broad range of substances, as it circulate what is injected ... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog Post...transcriptional regulator without making any functional changes to Cas9. For instance, when native (nuclease-competent... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...to K‐Ras and inhibit Sos‐mediated activation." Angewandte Chemie 124.25 (2012): 6244-6247. PubMed PMID:... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...The backbone modifications listed can refer to changes made to the original backbone by the depositing... -
Genome-wide Screening Using CRISPR
TypeBlog Post...scaled up for genome-wide screening due to the broad range of potential target sequences and ease of generating... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post... entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8):2636-43.) PubMed... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...to expand the toolkit. We have included a wider range of terminators than are commonly used as we have... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol... or Klenow DNA Polymerase I for 3′ overhang removal and 5′ overhang fill-in. If you are using blunt ends...amount of DNA in the reaction, incubation time can range from 45 mins to overnight. For diagnostic digests...compatible sticky ends, you will need to fill in the overhangs and conduct a blunt end ligation. Use T4 DNA Polymerase... -
Colony Formation Titering Assay
TypeProtocol...fresh media containing selection reagent Days 3–14: Change media as needed Days 14–18: Stain cells and count...empirically determined. Treat the target cells with a range of doses of antibiotic. Determine the minimum concentration...presence of selective reagent for 12 days with media exchanges every 3–4 days. Colonies were then stained with...presence of selective reagent for 12 days with media exchanges every 3–4 days. After 12 days of selection, no... -
Tips for arabidopsis transformation
TypeBlog Post...Agrobacterium all over your gloves, so be sure to change gloves between constructs if you are transforming... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...sgRNA targets. CRISPRi is effective in a narrow range around the transcription start site (TSS). The Root...