...to expand the toolkit. We have included a wider range of terminators than are commonly used as we have...

...transformation/replication process. LIC employs long overhangs to form a stable association between fragments...exonuclease functions, T4 DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on...recognition sequence. This can create multiple distinct overhangs with a single enzyme, and remove the restriction...in subsequent reactions. Step 3: Create Vector Overhangs Treat the linearized vector with T4 DNA polymerase...accomplished by gel purification . Step 5: Create Insert Overhangs Treat your purified PCR product with T4 Pol in...

...their science, meaning that they will heavily evangelize for the mode of communication they connect best...

...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic...

... you’re more of a leader than you thought! When change happens at your organization, when your boss needs...

... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs...

...e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging...

...Agrobacterium all over your gloves, so be sure to change gloves between constructs if you are transforming...

...sgRNA targets. CRISPRi is effective in a narrow range around the transcription start site (TSS). The Root...

...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...

...red lines), which maintains structural epitopes (orange region), but it can block epitopes hidden within...

...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality...

... your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life...

...PMID: 33230293 Yang, S., Zhang, Y., Xu, J., Zhang, J., Zhang, J., Yang, J., Jiang, Y., & Yang, S. (2021)....28931002 Cheng, A. W., Wang, H., Yang, H., Shi, L., Katz, Y., Theunissen, T. W., Rangarajan, S., Shivalila, ...Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng...PMID: 32217751 Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch...e04766. PMID: 25497837 Ma, Y., Zhang, J., Yin, W., Zhang, Z., Song, Y., & Chang, X. (2016). Targeted AID-mediated... Q., Xie, Z., Bai, M., Dong, R., Tang, W., Xing, Y., Zhang, H., Yang, S., Chen, L., Bartolomei, M. S.,.... You can therefore change the genomic target of the Cas enzyme by simply changing the target sequence...

...ensures the accuracy of your experiment and any changes to the amount you are dispensing can negatively...adjustment ring right below the plunger. This ring changes the pipette volume. Below that is the tip ejector...boxes that the tips come in often indicate a volume range that the tip can hold. This should give you an idea...without cross contamination as long as the tip is changed between samples. Tips can come loose in a bag, ...accurately and precisely is important since even small changes in the volume could affect your experiments. Beyond...

...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...

...targets (ones that are less likely to mutate or change in order for the bacterium to survive). Check out...

...may offer a lot of pointed feedback or abruptly change needs and expectations. Such situations can get...

...of reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work...

...touch and reach back out when you are looking to change positions in the future. Anyone looking for work...