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Showing: 741 - 760 of 946 results

  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...cells for long periods of time. This reduces the chance of integration at random locations that could cause..., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, D. P., Aryee, M. J., Joung, J....

  2. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ...time consuming to produce, so most labs opt to purchase adenoviruses from dedicated adenoviral production...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...

  4. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since the discovery... the easy part; using them can be a bit more challenging. Addgene’s free educational resources help scientists...

  5. Cloning Mammalian Cells with the Agarose Method

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    Blog Post
    ...individual cell clones with different expression characteristics from a population of transfected clones. The...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

  6. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...Knockout plasmids Homologous recombination is a mechanism to accurately repair harmful double stranded breaks... breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of DNA...

  8. Tips to Make the Most of a Scientific Conference

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    Blog Post
    ...attending on the larger side? At large conferences, chances are that several talks will be going on at once... for myself like meet ten new people each day. Exchange business cards. When you’re done networking for...

  9. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab. Recent findings...candidates using a synthetic human scFV library and characterized them by ELISA, homogeneous time resolved fluorescence...

  11. Academic vs. Industry Postdocs

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    Blog Post
    ...setting and no manager will ever be perfect, but the chances of getting a good manager do tend to be higher ...in-depth discussion about your project might be challenging. In an academic environment, surrounded by lab...

  12. Transferable Skills: Negotiation

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    Blog Post
    ...are they different from mine? What equipment purchases do we prioritize in a lab with both electrophysiologists...Collide: Managing Successfully Across Cultures by Richard D. Lewis Additional resources on the Addgene...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ...heard of another double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ...of a single microhomology sequence. For more mechanistic details on HDR and NHEJ, please see the linked...

  14. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...The underlying method was developed in 1902 by Richard Zsigmondy and Henry Siedentopf to enhance the microscopic...using cameras with electron multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor...

  15. Great Results Start with Great Standard Curves

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    Blog Post
    ...final readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or...dilution series, use consistent dilution factors, change pipette tips regularly, and mix samples very well...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...γ-Retrovirus Guide References Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex...

  17. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post
    ...Cancer Data Science team at Broad, they were able to change their analysis to use a corrected genome map. Now...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...occurring animal model, the RPE65−/− dog, was characterized and found to suffer from early and severe visual.... PubMed PMID: 29983335. 9. Day TP1, Byrne LC, Schaffer DV, Flannery JG, 2014. Advances in AAV vector ...

  19. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...identification of DNA binding proteins, this method can characterize nucleic acid-nucleic acid interactions at a ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...
Showing: 741 - 760 of 946 results