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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 810 results for: mal

Showing: 741 - 760 of 810 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors, but just as effective and...

  2. How to Be an Excellent Trainee

    Type
    Blog Post
    ...demonstrating it. Requests like this are both very normal and very helpful; don’t be afraid to ask! Especially...

  9. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...fragments of linearized DNA migrate slower than smaller linearized fragments. You can separate your backbone...

  15. CRISPR Guide

    Type
    Guide
    ...Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very precise edits of ...double-stranded DNA plasmid. Small ssDNA oligos often work well for small edits like a single-point mutation...Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9 Fusion...missing sequence, and is more commonly used for small precision edits. NHEJ directly ligates the break...efficient in repairing DSB, it frequently causes small nucleotide insertions or deletions (indels) at the...modification ). In most cases, NHEJ gives rise to small indels in the target DNA that result in amino acid... handful of bases, or even a single base. These small precision edits can be done via HDR, base editing...

  16. Antibody Guide

    Type
    Guide
    ...methods: in animals, in cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies...Antibodies produced in animals make use of the animal’s immune system. The antigen of interest is introduced...introduced to the animal, which then mounts an immune response to it. Once the immune response has developed...antibody. Monocultures of B cells isolated from animals immunized as described above are used to create... protein chains: two large heavy chains and two small light chains. These four domains come together to...only produce one variable region. Isotypes The mammalian immune system produces five classes of antibodies...antibodies are typically collected directly from animals and contain antibodies targeting various epitopes...

  17. Promoters

    Type
    Guide
    ... TATAAT. Proximal Promoter Further upstream from the core promoter you will find the proximal promoter...Constitutive Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter from... Constitutive Strong hybrid mammalian promoter PGK Constitutive Mammalian promoter from phospholycerate...portions that make up a promoter: core promoter, proximal promoter, and distal promoter. Below describes...Promoter The core promoter region is located most proximal to the start codon and contains the RNA polymerase...contains many primary regulatory elements. The proximal promoter is found approximately 250 base pairs...called the distal promoter which is upstream of the proximal promoter. The distal promoter also contains transcription...

  18. Molecular Biology Reference

    Type
    Guide
    ...order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...enables the preparation of more managable (i.e., smaller) DNA fragments. In 1952, Joshua Lederberg coined...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...carry genes and can replicate independently from chromosomal DNA. Although they can be found in archaea and...relationship with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division...plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs) to enable easy manipulation...fragments can be inserted. When a plasmid exists extrachromosomally in E. coli , it is replicated independently...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Adeno-Associated Viruses (AAV) are small, non-enveloped viruses containing a small, linear single-stranded DNA... indicating the optimal serotype(s) for transduction of a given organ. Tissue Optimal Serotype Central...genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses that belong to the Parvoviridae...limited by the physical space available inside of the small AAV capsid and directly correlates to the size of...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous...period of years without damaging the host cell. Episomal stability allows for long-term transgene expression... deliver genes to a wide range of tissues with minimal immune response, and the low risk of insertional...

  20. Plan Your Experiment

    Type
    Guide
    ...Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian cell line...Although we will use the example of CRISPR/Cas9 in mammalian cells, many of these principles apply to using...dCas9-KRAB is more effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression...expression systems and variables for using CRISPR in mammalian cells. Some of the variables include: Species ...Expression System Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive...expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell... ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes) Transient or stable expression of ...
Showing: 741 - 760 of 810 results