All in a Twist: dsRNA
Type
Blog Post
...Endogenous sources of duplexed RNA
DNA:RNA hybrids
Sometimes ssRNA just needs a buddy, and even DNA will suffice...
We narrowed to 850 results for: met
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CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...from Cas9 and almost all other Cas enzymes is its method of DNA cleavage. Instead of cleaving DNA at a defined... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...Protein A or Protein G purification is one common method. These proteins bind the IgG heavy chain. Running... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...multiple) genetic perturbations in single cells. Methods to scale up multiplexed CRISPR systems for high-throughput... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...cumbersome, can lower on-target efficiency, and sometimes even increase off-target effects, respectively... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post... of questions surrounding cancer development, metastasis, and drug resistance, Bhang says “the [drug] ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...al. Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016. PubMed PMID: 27110581. PubMed Central... -
Lab Automation at Addgene
TypeBlog Post...automate and optimize more protocols in the future - something that will be key for our future growth. One of... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... may enter “donor template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis,... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...available destination vectors for various delivery methods, along with compatible inserts. All destination... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...chromosomes recombine during meiosis, creating gametes that contain a mix of genetic material from each... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post...to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting (FACS... -
Hot Plasmids - October 2020
TypeBlog Post...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post... does. Her parents told her that if she found something she loves to do, she would never work a day in... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...engineered. Fig. 1: Antibody production methods NeuroMab and others have an extensive library... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these... -
A Look at Addgene's QC Process
TypeBlog Post...the plasmid to stop functioning properly or be something completely different than what was originally ... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...Krebs, A., Schübeler, D., Hajdú, R.I., Lukáts, Á., Németh, J., Nagy, Z.Z., Wu, K., Wu, R., Xiang, L., Fang... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...the Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona...