Hot Plasmids - November 2023
Type
Blog Post
...undesired edits (indels, etc.), while PE6c and PE6d were most efficient at complex edits, such as long or highly...
We narrowed to 780 results for: mos
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Viral Production at Addgene
TypeBlog Post...This article was written by multiple Addgenies and most recently updated by Ina Ersing in August 2023. ... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...first try to produce GVs in their own lab using the most reliable method, which is the transient cotransfection... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...type of readout allows for nucleic acid detection almost anywhere as lateral flow strips are easy to transport... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...same replication control machinery within the cell. Most plasmids encode a negative regulation system involving... -
An Inside Look into Addgene's Dev Team
TypeBlog Post... third party libraries and any security updates. Most of the time, updating these libraries is straight... -
CRISPR Activation: A Practical Guide
TypeBlog Post...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post... need to sequence your PCR to visualize an edit. Most dual guide systems generate a deletion large enough... -
Tips for Getting a Faculty Position
TypeBlog Post...research. Still a Grad Student? Get Tips on Getting the Most out of the Process Get more tips from the full eBook... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid in bacteria (Ahmed... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...the orientation of the BbcVI restrict site matter? Most of the time no, but there are 2 instances where ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...can be difficult to compare their performance, as most translation initiation sequences are optimised for... -
Site Directed Mutagenesis by PCR
TypeBlog Post...Consequently, site directed mutagenesis is one of the most popular approaches for introducing minor modifications... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...recombination, discovered in the 1980s, is one of the most important ways to spatially and temporally control... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...epigenetic modifiers available from Addgene. For most of these constructs, catalytically dead modifiers... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...fluorescent protein. FRET can be measured by exciting the most blue-shifted fluorescent protein (donor) and measuring... -
Plasmids 101: Restriction Cloning
TypeBlog Post...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...traction in recent years. The goal is to engineer the most miniscule parts of life, like proteins, to study...