We narrowed to 942 results for: omp
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TypeBlog Post...important in experiments in which viability is being compromised by introduction of foreign RNAs or immunity itself... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...
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Antibodies 101: Secondary Antibodies
TypeBlog Post...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...related to addiction and pain. Once these are completed, Hubbard will image these neurons using GCaMP7... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post... but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor. This may...Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope... -
Addgene: The First Twenty Years
TypeBlog Post...Addgene! Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids, was shared with researchers ... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post... Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 projects per year... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding..., GFP linked the two GBPs together to create a complete transcription factor, activating luciferase transcription... -
The Future of Research Symposium Boston 2015
TypeBlog Post... about how the system operates and functions” “Complete lack of information on number of postdocs” “Postdocs...Harvard’s Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post... Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI. ...-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse... -
Design Tips for Prime Editing
TypeBlog Post...extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase (RT...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post.... The donor and acceptor fluorophores must be compatible, such that the emission spectrum of the donor...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ... -
Hot Plasmids - October 2020
TypeBlog Post...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not fluorescent on... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...multiplexed analysis of cells pre-treated with a compound of interest. Lentiviral vectors Popular Lentiviral...modular and flexible system can be used to construct complex lentiviruses for modification of mammalian cells... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...development, recognition and migration, the immune compromise observed in CDG patients is not surprising. However... into functional multi-subunit macromolecular complexes, but a serendipitous discovery led them to investigate... -
Hot Plasmids February 2024
TypeBlog Post...variant has similar photostability and brightness compared to the original, though its maturation is even...cell-specific tropism, with slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post... for us all. For example, implementation and compliance for the FLSA changes, including finding money...postdocs nationwide. On this level, FoR is currently compiling a database of institutional responses to the FLSA... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...required to produce your fiber optic implant. Companies such as Thor Labs and Precision Fiber Products...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow... -
Common Injection Routes in Mice
TypeBlog Post... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...