Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 1,055 results for: sta

Showing: 741 - 760 of 1055 results

  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...limitations because it turns a dynamic process into a static measurement and it provides limited information...mammalian dual-emission autophagy biosensor. Its resistance to lysosomal proteases is key to its pH-dependent...in acidic environments, while mRFP is relatively stable and fluorescences even at the acidic pH found in...

  2. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...biology tool. These systems allow researchers to understand bacterial gene clusters that produce useful natural...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...base editors to disrupt gene function by targeting start codons, splice acceptor and donor sites, and introducing...

  4. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...the 3rd edition has now been released), and have started providing recorded interviews with Addgene community...and actionable content in the coming years as we stay on top of the many developments in science and the...give researchers the background they need to get started using plasmids and the revolutionary CRISPR technology...

  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...about biology in high school, it wasn’t until I started working in my first molecular biology lab in college...into pellets (FD-CF pellets), leaving them shelf-stable until water is added and protein production proceeds... 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...

  6. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...help me get the engagement I wanted. I therefore started planning a game that would force my students to...and strategy skills to apply those concepts and understand how therapeutic strategies work. Find Addgene...Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection Markers) and one “Transfer...

  7. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    .... In her graduate work in Michael Lin’s lab at Stanford, Xin Zhou greatly expanded that list by using ...graduate student in Thomas J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based...throughout her training and wants to make her work understandable to people both inside and outside the scientific...

  8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... treated HeLa cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase...retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...vitro and in living organisms to better study and understand these diseases and disorders. You can find the...

  9. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...their research into both novel and previously established ethical issues raised around mitochondrial replacement...reasonably safe and effective. We do that through a standardized process of good research practice: computer ..., in vitro research, animal models, and several stages of human clinical trials. This model also demonstrates...

  10. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. Create yellow...modified form of the cytosolic C-terminal domain of Stargazin including a terminal PDZ ligand. The researchers...PMCID: PMC4469972 Marvin, Jonathan S., et al. “Stability, affinity and chromatic variants of the glutamate...

  11. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...hACE2 transgenic mouse strain, for example, is sustained by the availability of this strain from The Jackson...Laboratory. Originally developed and characterized by Stanley Perlman, of the Carver College of Medicine and ...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

  13. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered... in 1985, luciferase assays have become a gold standard in gene expression analysis to investigate the...

  14. Clear and Accessible Writing

    Type
    Blog Post
    ...simple goal… and we had no idea how much our understanding of what accessibility is would change over the...information? This can be physical or digital. For instance, you would present information differently in ...paper accessible to even more people.  Whether you start small with alt text or immediately dive into applying...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... turn it down, knock it out, etc. It’s how we understand what genes do! Viruses have several options to...of each variable being manipulated. Thus, the adjustable nature of viral libraries makes them the ideal...services and even a Viral Vectors eBook to get you started. Ready to plan your next (or maybe first!) viral...

  16. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...three times, to opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited.... Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    ...nanobody to its target stabilizes the TurboID-nanobody protein and allows TurboID to start labeling proteins...biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...promoter using the AAV-BR1 capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions.... We are also adopting ddPCR, which does not a standard curve and somewhat more reliable quantitation....outlier for PHP.eB (1x1011 - 1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top...

  19. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...If you’re just getting started using antibodies in your experiments, you may be curious about all of ...antibodies depends on a variety of factors. Understanding the advantages and disadvantages of monoclonal...AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS...

  20. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...begin and end with the same steps as screens – they start with manipulations (CRISPR, etc.), and finish with...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...answer your experimental question. Now that you understand the difference between the two, it’s time to ...
Showing: 741 - 760 of 1055 results