...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall... curves in their day-to-day work. Indeed, viral vector production facilities frequently use qPCR with ...with each step of the series diluted by the same factor. For example, Figure 1 depicts a 2-fold dilution... standard curves vary depending on a number of factors, including the user, equipment, assay incubation...measure.  Dynamic range depends on a number of factors, including the dilution series of the standard ...standard curve dilution series with lower dilution factors. If the unknown sample’s measurement is too high...assays you will be using ahead of time to see what factors may impact the dynamic range of the assay. Try ...

... the notation works? The + operator denotes intersection and the – operator denotes set difference. That...inclusive markers of regulatory regions in the genome, including enhancers, promoters, insulators, and more. I...proteins”) for NANOG, an essential transcription factor in embryonic stem cells (ESCs). Your ChIP-seq finds...of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as...enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that this matter exists, but...criteria: no TTTTT allowed (this is a polymerase terminator), and no off-target effects for the identified...

...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create... concrete happen. You may have more willing collaborators than you realize. Being at a university provides...meetings and take minutes. Get to know your collaborators from other institutions personally on conference...relationship with the perspective of my potential collaborators in mind. Being at a university allows you to...with people at your organization. You can meet collaborators  for lunch, make a point of talking to them ... different countries). Here social media is a connector and virtual meeting place: You can follow interesting...as messages and keep you up-to-date on your collaborators’ professional developments without directly ...

...us. Use a well working vector system This allows you to quickly assemble vectors and test different ideas...MoClo system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo...to quickly screen and investigate transcription factor candidates and the regulation of genes in the biosynthetic... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...system you can easily and quickly create customized vectors. For more information on MoClo for plants, check...

...-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Darrell Kotton is the director of the Center for Regenerative Medicine at Boston University Medical School... Darrell recently joined the Addgene Board of Directors where he hopes to help keep us focused on our ...Darrell's goals as a member of the Addgene board of directors - increasing open source sharing and helping us...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...

...they do so is through G protein-coupled receptors (GPCRs), receptors that play many roles in signaling processes...which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET is that the... between a luminescent donor and a fluorescent acceptor. TRUPATH uses an updated version of BRET called...constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs...non-uniform across the transducerome for a specific receptor-ligand pair. They further used TRUPATH to analyze...Mitaku S (2002) Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Letters...

... referred to as “vectors” or “constructs.” To insert a gene of interest into a vector, scientists may ...plasmid with its elements described below.   Vector Element Description Origin of Replication (ORI...the target gene. Vital component for expression vectors: determines which cell types the gene is expressed...Regardless, once the cloning steps are complete, the vector containing the newly inserted gene is transformed...Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular environment...about molecular cloning, how to choose a plasmid vector, molecular biology tools and references, and how...

...upon an indicator they developed and published in 2010 for visualizing the transcription factor cAMP-responsive...Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the...Griesbeck is motivated by a particular interest in monitoring the activity and biochemistry of living neurons...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...while in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple... variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new colors might now enable...

..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform... study has just been uploaded to the preprint repository BioRxiv. This example clearly illustrates the...  Figure 2: When a researcher needs a collaborator for a specific task, Crowdfight will: 1) Find...researchers, such as postdocs and principal investigators. They also come from many different scientific...website. Once curated by our in-house team of collaborators, the requests were forwarded to our volunteer...IgnacioAmigoH     María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...

...Scientific Illustrator Plant Breeder Zoological Worker Conservationist Documentary Director Parasitology...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor...Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer     Fieldwork...Bioinformatician Science Writer Grant Writer Journal Editor Science Marketer Software Developer Learn Science..., and how many jobs are normally available (as sectors such as forensics currently have too many applicants...hobbies and volunteer experiences, as well as work history can really show that you have some of the abilities...