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We narrowed to 532 results for: abo.3

Showing: 381 - 390 of 532 results
  1. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ... epitopes that will likely bring about unwanted immunogenicity. The 3-D models can be applied to corroborate...limitations. Using methods together can inform decisions about in vivo application and clinical testing. This would...to drugs: adverse reaction terminology. WHO Collaborating Centre for International Drug Monitoring, Uppsala...
  2. K. phaffii: Rising to the Occasion

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    Blog Post
    ... Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose...pombe have dominated the research scene. But what about the other yeasts – in the biomedical world, are ...pharmaceutical companies (along with some research laboratories) have picked up the system for some of the uses...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...
  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...
  4. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post
    ... lab E. coli K12 strains such as DH5alpha contain 3 methylases that recognize and methylate different ...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...
  5. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...development since its inaugural year in 2012. That’s just 3 to 4 years ago. It is a bubble of innovation (a piñata...PMID: 23873081. PubMed Central PMCID: PMC3969858. 3. Doench, John G., et al. "Rational design of highly...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...
  6. Hot Plasmids - January 2023

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    Blog Post
    ...cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...
  7. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...). Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how...
  8. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...guide RNA, (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite...sites with A's, producing C → A edits. Figure 3: Mechanism of cytosine transversion editors. Red arrows...still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion editors...CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: Prime Editing...
  9. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents developed during the SARS-...the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...
Showing: 381 - 390 of 532 results