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We narrowed to 971 results for: lars;

Showing: 381 - 390 of 971 results

  1. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...role of individual genes or entire pathways in a particular disease process. While CRISPR is certainly not... mutant cells to identify genes involved in a particular phenotype. CRISPR can be readily scaled up for...fluorescence-based cell sorting and screened for a particular phenotype. For example, mutant cells can be used...cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid...targeting the same gene can be strong evidence that a particular gene is actually important for a given phenotype...Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002/cpmb.8 ...

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...results. The default setting is “Optimize for Highly similar sequences (megablast)”, which is very fast and ...often unreliable, we suggest using the “Somewhat similar sequences (blastn)” program for blastn so that ...your sequencing trace file. Unlike megablast, the regular blastn program uses a smaller word size and lower...protein sequences (nr)” database as it contains the largest number of protein sequences. Just below the BLAST...options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often have repeated...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...transistor-like digital genetic switches that enable cellular computing. These logic gates built from transcriptors...of promises to not assert property rights for particular engineered genetic sequences; but it’s only the...computer languages are free to use. Of the 15 most popular computer programming languages, 12 out of 15 are...are now available exist within the context of the larger bioeconomy, and that it’s been 40 years since the... on the domestic market generates $300 billion dollars every year. That's two percent of the U.S. gross... we anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward...

  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... Boyden Lab, ExM has been steadily growing in popularity with an abundance of protocols and examples of...not something you find in the average cell and molecular biology lab, and EM is limited in the number of...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid..., Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-fold Robust... Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures using expansion microscopy (U-...Wijesekara P, et al (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat...

  5. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous...fortunate enough to apprentice with top scientists to larger groups of trainees at an earlier point in their...protein with easily measured activity, MDH is particularly amenable to in-class labs. Provost ultimately... at the American Society for Biochemistry and Molecular Biology Annual Meeting. Some of my students published...and green fluorescent protein. Biochemistry and Molecular Biology Education. 38(5):317-323. Knutson, K.,...dehydrogenase as a research model. Biochemistry and Molecular Biology Education. 38(5):324-329. Kowalski, J....

  6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...information while using less resources and are particularly useful when trying to screen a limited number... libraries have been shown to outperform other popular CRISPR libraries (see below). Let’s take a look...Brunello library was more effective than other popular CRISPR libraries in the depletion of essential ...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...about the advantages of pooled CRISPR libraries for large-scale functional screens Read this blog post about...

  7. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...specializes in developing novel technologies to analyze molecular mechanisms of genome functions such as epigenetic...transcription and epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions, ...isolate specific genomic regions while retaining molecular interactions. For this purpose, we developed the...merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred requests, and...been very difficult for us to respond to such a large number of requests by ourselves without Addgene.... experiences in research labs. What are some similarities and differences you have observed as you have...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...fluorescent proteins have become a mainstay in molecular biology. Scientists can easily utilize GFP-containing...biosensors has been designed to detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations...can use light to detect, measure, and control molecular signals, cells, and groups of cells in order to...applications for GFP. It has also been used to identify particular cell populations in drug screens, to visualize...strand break repair, and to label pathogenic intracellular microbes to visualize host/pathogen interactions...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...commonly used to create functional knockouts or large deletions, the ability to analyze and interpret ...which of the multiplexed sgRNA was involved in a particular edit, and which type of edit was produced.   ...characterizes the sequence and abundance of each particular indel.   For a complete guide to ICE analysis...Typically, a yellow check mark indicates that a particular parameter was automatically adjusted to generate...+F or Command+F functionality to search for a particular sequence or name. You can perform more in-depth...Contributions, Indel Distribution and Traces - provides particular details about the indel profile of the edited...

  10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Bird A (2020) The Molecular Basis of MeCP2 Function in the Brain. Journal of Molecular Biology 432:1602... consolidated and centralized a collection of molecular biology tools so that both veteran and new Rett...severity. This exciting finding injected hope that a similar reversal may be possible for children and adults...RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and promising results for...Apparent Toxicity after Intracisternal Delivery. Molecular Therapy - Methods & Clinical Development 5:106...
Showing: 381 - 390 of 971 results