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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,041 results for: ins

Showing: 761 - 780 of 1041 results

  1. Tips for Technical Support Calls

    Type
    Blog Post
    ...last digit: An answer should come soon, but not instantly. Addgene tries to get back to customers within... Eric J. Perkins...

  2. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... now specifically drives expression of mCherry instead of BFP. Beyond switching fluorophores, why is FLEx...optogenetic reporter would ensure that the reporter remains silent until a cell or tissue-specific Cre is provided...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...., Pawlita, M., Ferrari, F., Samulski, R., & Kleinschmidt, J. (1999). Titration of AAV-2 particles via...

  4. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...transcription and chimeric efforts make a precise edit or insertion via a chemical modifier. We used these categories... plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age) are requested...

  5. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...light to distinguish one fluorophore from another. Instead of using a single (primary) detector to identify...narrow band, to discern one marker from another. Instead of compensation, which relies on user adjustment...

  6. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...plasmid of interest, for detecting the presence of an insert or piece of DNA in a sample such as in colony PCR...into approximately 20,000 droplets. Each droplet contains the material required for PCR amplification. Following...

  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...questions. Practice your presentation for both instances. If your poster material is nearing publication...Connect with people on LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...time to produce beta-lactamase prior to division, instead of immediately experiencing the effects of the ... capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories, 11...

  12. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...information. Personally, I would much rather read the instructions than watch a how-to video. My dad, on the other...love it and others will avoid it like the plague. Instead of looking for The One Method to Rule Them All,...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...including casual request via email, in person, or instant messaging but we as scientists often prefer data...failing in many academic journal clubs where institutional inertia drives the meeting rather than interest...

  16. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...run and the long run and so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes...tasks – try to resist the impulse to control by insisting things always be done your way. Be patient with...

  17. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...you can either order a DNA pooled library, which contains a collection of plasmids in a single tube, or ...lentiviral CRISPR libraries. Protocols from the Broad Institute- A variety of protocols and guides regarding pooled...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection with ...Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...is not amenable to FACS.  Thus, the researchers inserted human CD8, a gene that encodes a surface protein...yourself whether gene overexpression is necessary (instead of gene knockout or knockdown, for which there ...
Showing: 761 - 780 of 1041 results