We narrowed to 960 results for: APP

...traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide enough...with the start codon or tag sequences (where appropriate). The primer length is dependent on the T4 Pol...

...fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal...part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short to make its...

...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mL of DMEM Complete to a 15 mL conical and an appropriate amount of 25 mM chloroquine to the conical so...

.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. Incubate the cells for ~2 weeks. ...replace it with fresh DMEM complete containing the appropriate antibiotic. When the untransduced (negative control...

... P1000. The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10...,000 µL Each pipette should be used with the appropriate tips, which are made in a number of sizes and...

...want a recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each...should also verify that you are plating on the appropriate antibiotic and try varying the amount of recipient...

... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis buffer. Pro-Tips The volume...

...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times. Lightly...

... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

... Western blot and share tips for this tricky application.    You can check out all of our protocols ...

...found on our Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists...

...introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the Addgene blog ...

...   We love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall...

... deposit. However, with all the great science happening everyday, it’s not possible for us to reach everyone...

...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...

... information on the basics of viral vectors, applications, and some new resources available from Addgene...

... Getting a Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware...

...post-holiday routines, why not take a little time to appreciate the humor in science and in the lab? Addgene ...

...Cytology group from the University of Amsterdam. Happy Holidays from the Molecular Cytology group! @EikeMahlandt...