...complete: Prepare 7 microcentrifuge tubes with the appropriate amount of media volume listed in the chart below...tube. Add 4 µL of the 25 ng/µL samples to the appropriate PCR tubes. Pipette back and forth 10 times to...the average infectious titer obtained from the appropriate dilutions to calculate the final infectious titer...

...within the entire construct. Is the primer an appropriate distance from the target? The primer should be... should I do? Check your trace file first; the apparent mismatch/mutation may be the result of a mis-called...

...in place. Turn the water bath on and set the appropriate temperature as described in your protocol. How...in step 3. You will also need to maintain the appropriate water level in the water bath so before use, ...

...traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide enough...with the start codon or tag sequences (where appropriate). The primer length is dependent on the T4 Pol...

...fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal...part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short to make its...

...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mL of DMEM Complete to a 15 mL conical and an appropriate amount of 25 mM chloroquine to the conical so...

.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. Incubate the cells for ~2 weeks. ...replace it with fresh DMEM complete containing the appropriate antibiotic. When the untransduced (negative control...

... P1000. The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10...,000 µL Each pipette should be used with the appropriate tips, which are made in a number of sizes and...

...want a recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each...should also verify that you are plating on the appropriate antibiotic and try varying the amount of recipient...

...written and video protocols for a wide range of applications in molecular biology, plasmid cloning, and virus...

...specific binding to other proteins. Antibody-based applications, which often use antibodies conjugated to a ...

... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis buffer. Pro-Tips The volume...

... Western blot and share tips for this tricky application.    You can check out all of our protocols ...

...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times. Lightly...

... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

...found on our Protocols page and YouTube page.  Happy learning, and welcome to the lab bench, new scientists...

...introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the Addgene blog ...

...   We love the way the names Aavery, Addy, and Cappy play on viral vector terms. And, the name Kendall...

... deposit. However, with all the great science happening everyday, it’s not possible for us to reach everyone...

...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...